Detailed information of TA system TA229819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 250986..251623
• Replicon: plasmid pORIO1
• Accession: NZ_CP089967
• Organism: Cereibacter azotoformans strain ORIO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ORIO_RS20695
• Protein ID: WP_238823620.1
• Coordinates: 251216..251623 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ORIO_RS20690
• Protein ID: WP_044249817.1
• Coordinates: 250986..251216 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ORIO_RS20660 (ORIO_20660)	246581..246763	+	183	Protein_218	type II toxin-antitoxin system HicA family toxin	-
ORIO_RS20670 (ORIO_20670)	247603..247935	+	333	WP_238823743.1	type II toxin-antitoxin system HicB family antitoxin	-
ORIO_RS20675 (ORIO_20675)	248372..248911	-	540	WP_238823618.1	hypothetical protein	-
ORIO_RS20680 (ORIO_20680)	249234..249998	+	765	WP_238823619.1	response regulator transcription factor	-
ORIO_RS20685 (ORIO_20685)	250198..250818	+	621	WP_011910905.1	LuxR family transcriptional regulator	-
ORIO_RS20690 (ORIO_20690)	250986..251216	+	231	WP_044249817.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ORIO_RS20695 (ORIO_20695)	251216..251623	+	408	WP_238823620.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ORIO_RS20700 (ORIO_20700)	251876..252277	+	402	WP_238823621.1	transposase	-
ORIO_RS20705 (ORIO_20705)	252572..253798	-	1227	WP_238823745.1	type II toxin-antitoxin system HipA family toxin	-
ORIO_RS20710 (ORIO_20710)	253801..254112	-	312	WP_101339567.1	helix-turn-helix transcriptional regulator	-
ORIO_RS20715 (ORIO_20715)	254868..255113	-	246	WP_011910894.1	hypothetical protein	-
ORIO_RS20720 (ORIO_20720)	255110..255547	-	438	WP_238823622.1	putative toxin-antitoxin system toxin component, PIN family	-
ORIO_RS20725 (ORIO_20725)	256116..256475	+	360	WP_044249727.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	glf / glf	1..309984	309984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14989.29 Da
• Isoelectric Point: 7.3591

>T229819 WP_238823620.1 NZ_CP089967:251216-251623 [Cereibacter azotoformans]
MLRYMLDTNILIYTIRNRPGEVRVKFEAHHGTMCISTVTAMELIYGAQKSQAVERNMAAVEGLLARLDILDLDLAAAQHA
GEIRAQLARAGTPIGPFDVMIAGHARSRGLVLVSNNTREFERVDGLRLENWVQTS

Nucleotide

• Length: 408 bp

>T229819 NZ_CP124523:c56671-56366 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8704.63 Da
• Isoelectric Point: 4.4091

>AT229819 WP_044249817.1 NZ_CP089967:250986-251216 [Cereibacter azotoformans]
VDRGSVFMNNRTQAIRLPKSVALPEGTRSVDIVRSGRAWLIVPSQDSWAQWFDEVPSSEDFMERREQPDADERGDL

Nucleotide

• Length: 231 bp

>AT229819 NZ_CP124523:c56891-56673 [Salmonella enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA