Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 20527..21263 | Replicon | plasmid pKP403-2 |
| Accession | NZ_CP089961 | ||
| Organism | Klebsiella pneumoniae strain KP403 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | L7SZ15 |
| Locus tag | LVQ74_RS26715 | Protein ID | WP_003026803.1 |
| Coordinates | 20781..21263 (+) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | L7SZ29 |
| Locus tag | LVQ74_RS26710 | Protein ID | WP_003026799.1 |
| Coordinates | 20527..20793 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LVQ74_RS26665 (LVQ74_26620) | 16589..16951 | - | 363 | WP_004152100.1 | arsenite efflux transporter metallochaperone ArsD | - |
| LVQ74_RS26670 (LVQ74_26625) | 17001..17351 | - | 351 | WP_004152101.1 | As(III)-sensing metalloregulatory transcriptional repressor ArsR | - |
| LVQ74_RS26675 (LVQ74_26630) | 17709..17978 | + | 270 | WP_004152102.1 | hypothetical protein | - |
| LVQ74_RS26680 (LVQ74_26635) | 17966..18541 | + | 576 | WP_004152103.1 | hypothetical protein | - |
| LVQ74_RS26685 (LVQ74_26640) | 18572..19066 | + | 495 | WP_004152104.1 | DNA-binding protein | - |
| LVQ74_RS26690 (LVQ74_26645) | 19110..19478 | + | 369 | WP_004152105.1 | hypothetical protein | - |
| LVQ74_RS26695 (LVQ74_26650) | 19512..19715 | + | 204 | WP_004152106.1 | HHA domain-containing protein | - |
| LVQ74_RS26700 (LVQ74_26655) | 19764..20021 | + | 258 | WP_004152107.1 | hypothetical protein | - |
| LVQ74_RS26705 (LVQ74_26660) | 20097..20351 | + | 255 | WP_004152108.1 | hypothetical protein | - |
| LVQ74_RS26710 (LVQ74_26665) | 20527..20793 | + | 267 | WP_003026799.1 | DUF1778 domain-containing protein | Antitoxin |
| LVQ74_RS26715 (LVQ74_26670) | 20781..21263 | + | 483 | WP_003026803.1 | GNAT family N-acetyltransferase | Toxin |
| LVQ74_RS26720 (LVQ74_26675) | 21471..22817 | + | 1347 | WP_077253535.1 | ISNCY family transposase | - |
| LVQ74_RS26725 (LVQ74_26680) | 22866..23261 | + | 396 | WP_004143398.1 | helix-turn-helix domain-containing protein | - |
| LVQ74_RS26730 (LVQ74_26685) | 23409..24574 | - | 1166 | Protein_33 | IS3 family transposase | - |
| LVQ74_RS26735 (LVQ74_26690) | 24751..25713 | - | 963 | WP_004152113.1 | zinc metalloprotease HtpX | - |
| LVQ74_RS26740 (LVQ74_26695) | 25700..26188 | - | 489 | WP_004152114.1 | phosphate-starvation-inducible PsiE family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..219770 | 219770 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17353.97 Da Isoelectric Point: 9.5822
>T229817 WP_003026803.1 NZ_CP089961:20781-21263 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
Download Length: 483 bp
>T229817 NZ_CP124522:c4495231-4494920 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG
Antitoxin
Download Length: 89 a.a. Molecular weight: 10147.59 Da Isoelectric Point: 4.5256
>AT229817 WP_003026799.1 NZ_CP089961:20527-20793 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
Download Length: 267 bp
>AT229817 NZ_CP124522:c4494919-4494629 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J2GNW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1Q8YL66 |