Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4472030..4472840 Replicon chromosome
Accession NZ_CP089959
Organism Klebsiella pneumoniae strain KP403

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LVQ74_RS21775 Protein ID WP_004178461.1
Coordinates 4472030..4472563 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LVQ74_RS21780 Protein ID WP_002887278.1
Coordinates 4472574..4472840 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ74_RS21770 (LVQ74_21735) 4470861..4471982 + 1122 WP_002887282.1 cupin domain-containing protein -
LVQ74_RS21775 (LVQ74_21740) 4472030..4472563 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LVQ74_RS21780 (LVQ74_21745) 4472574..4472840 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LVQ74_RS21785 (LVQ74_21750) 4472943..4474376 - 1434 WP_023159196.1 Cu(+)/Ag(+) sensor histidine kinase -
LVQ74_RS21790 (LVQ74_21755) 4474366..4475049 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LVQ74_RS21795 (LVQ74_21760) 4475221..4476606 + 1386 WP_023159195.1 efflux transporter outer membrane subunit -
LVQ74_RS21800 (LVQ74_21765) 4476624..4476968 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T229811 WP_004178461.1 NZ_CP089959:c4472563-4472030 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T229811 NZ_CP124522:3412093-3412311 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT229811 WP_002887278.1 NZ_CP089959:c4472840-4472574 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT229811 NZ_CP124522:3411691-3412065 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References