Detailed information of TA system TA022981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 1822804..1823369
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B4SAZ9
• Locus tag: PPHA_RS08465
• Protein ID: WP_012508432.1
• Coordinates: 1823025..1823369 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B4SAZ8
• Locus tag: PPHA_RS08460
• Protein ID: WP_012508431.1
• Coordinates: 1822804..1823028 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS08430	1819046..1820107	+	1062	WP_012508426.1	glycosyltransferase	-
PPHA_RS08435	1820141..1821337	+	1197	WP_012508427.1	glycosyltransferase family 2 protein	-
PPHA_RS08440	1821344..1821637	-	294	WP_012508428.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PPHA_RS08445	1821634..1821840	-	207	WP_012508429.1	addiction module protein	-
PPHA_RS08455	1822165..1822671	+	507	WP_012508430.1	hypothetical protein	-
PPHA_RS08460	1822804..1823028	+	225	WP_012508431.1	DUF433 domain-containing protein	Antitoxin
PPHA_RS08465	1823025..1823369	+	345	WP_012508432.1	DUF5615 family PIN-like protein	Toxin
PPHA_RS08470	1823395..1823631	+	237	Protein_1659	hypothetical protein	-
PPHA_RS08475	1823841..1824407	+	567	WP_041526793.1	hypothetical protein	-
PPHA_RS08480	1824416..1826869	+	2454	WP_012508435.1	tetratricopeptide repeat protein	-
PPHA_RS08485	1827057..1827290	+	234	WP_041526794.1	hypothetical protein	-
PPHA_RS08490	1827457..1827669	-	213	WP_041526492.1	hypothetical protein	-
PPHA_RS08495	1827650..1827931	-	282	WP_012508438.1	BrnT family toxin	-
PPHA_RS08500	1828073..1828300	-	228	WP_012508439.1	CopG family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-79)

Antitoxin

• Protein of unknown function DUF433 (8-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12721.59 Da
• Isoelectric Point: 5.1281

>T22981 WP_012508432.1 NC_011060:1823025-1823369 [Pelodictyon phaeoclathratiforme BU-1]
MIIWIDAQLSPSIAAWINRSYPNIQAQSLRSLDLQRADDLAIFDAAKHTEAVIKSKDSDFLKLVDQFGTPPQIIWITCGN
TSNNRMRAVLEKSLEKAVELIRSGEPIVEIGDKA

Nucleotide

• Length: 345 bp

>T22981 NC_011060:1823025-1823369 [Pelodictyon phaeoclathratiforme BU-1]
ATGATCATCTGGATTGATGCACAACTATCCCCCTCTATTGCTGCATGGATCAACCGCAGCTATCCGAATATTCAGGCACA
ATCACTGAGGTCTCTCGATCTTCAGCGAGCGGATGATCTTGCCATATTTGATGCCGCAAAACATACCGAAGCAGTGATCA
AGAGTAAAGATTCGGATTTCCTGAAACTTGTCGATCAGTTCGGCACGCCGCCTCAAATCATCTGGATCACTTGTGGTAAC
ACCTCGAACAACAGAATGAGAGCTGTTCTTGAAAAATCTCTTGAAAAGGCTGTGGAATTGATACGTTCAGGCGAGCCGAT
CGTGGAAATCGGTGACAAAGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8282.58 Da
• Isoelectric Point: 4.7376

>AT22981 WP_012508431.1 NC_011060:1822804-1823028 [Pelodictyon phaeoclathratiforme BU-1]
MTDWQKRITINPEQCGGRPCIRGLRIRVIDILDLLAAGLSSEEILDELPDLEKDDIQAALKYASRKLDHPVIAA

Nucleotide

• Length: 225 bp

>AT22981 NC_011060:1822804-1823028 [Pelodictyon phaeoclathratiforme BU-1]
ATGACTGACTGGCAAAAACGGATCACGATTAACCCCGAACAATGTGGTGGAAGACCATGTATTCGGGGATTGCGTATAAG
GGTAATTGATATCCTTGACCTGCTGGCGGCTGGTCTCAGCTCTGAAGAAATTCTGGATGAACTACCCGATCTCGAAAAAG
ATGATATTCAGGCTGCGCTGAAATATGCAAGCCGCAAACTCGATCATCCTGTCATTGCGGCATGA

Structures

Toxin

• AlphaFold DB: B4SAZ9

Antitoxin

• AlphaFold DB: B4SAZ8