Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2538699..2540287 Replicon chromosome
Accession NZ_CP089959
Organism Klebsiella pneumoniae strain KP403

Toxin (Protein)


Gene name hipA Uniprot ID W8UI36
Locus tag LVQ74_RS12505 Protein ID WP_002904792.1
Coordinates 2538699..2540021 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag LVQ74_RS12510 Protein ID WP_002904790.1
Coordinates 2540021..2540287 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ74_RS12490 (LVQ74_12480) 2533755..2536256 + 2502 WP_023158393.1 fimbria/pilus outer membrane usher protein -
LVQ74_RS12495 (LVQ74_12485) 2536244..2537248 + 1005 WP_023158392.1 fimbrial protein -
LVQ74_RS12500 (LVQ74_12490) 2537390..2538682 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
LVQ74_RS12505 (LVQ74_12495) 2538699..2540021 - 1323 WP_002904792.1 type II toxin-antitoxin system HipA family toxin Toxin
LVQ74_RS12510 (LVQ74_12500) 2540021..2540287 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LVQ74_RS12515 (LVQ74_12505) 2540553..2540879 - 327 WP_004184023.1 hydroxyisourate hydrolase -
LVQ74_RS12520 (LVQ74_12510) 2540876..2541376 - 501 WP_004184022.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
LVQ74_RS12525 (LVQ74_12515) 2541354..2542733 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
LVQ74_RS12530 (LVQ74_12520) 2543079..2544233 + 1155 WP_023158390.1 FAD-dependent urate hydroxylase HpxO -
LVQ74_RS12535 (LVQ74_12525) 2544214..2545137 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaSHV-76 mgtC / iroE 2363689..2606782 243093


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T229808 WP_002904792.1 NZ_CP089959:c2540021-2538699 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T229808 NZ_CP124522:c989052-988525 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT229808 WP_002904790.1 NZ_CP089959:c2540287-2540021 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT229808 NZ_CP124522:c989339-989049 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CEC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References