Detailed information of TA system TA022980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 937512..938107
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B4SF39
• Locus tag: PPHA_RS04450
• Protein ID: WP_012507681.1
• Coordinates: 937799..938107 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4SF38
• Locus tag: PPHA_RS04445
• Protein ID: WP_012507680.1
• Coordinates: 937512..937802 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS04425	935060..935413	-	354	WP_012507675.1	hypothetical protein	-
PPHA_RS04430	935930..936373	-	444	WP_012507676.1	hypothetical protein	-
PPHA_RS04435	936707..936985	-	279	WP_012507678.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PPHA_RS04440	936985..937212	-	228	WP_012507679.1	ParD-like family protein	-
PPHA_RS04445	937512..937802	-	291	WP_012507680.1	putative addiction module antidote protein	Antitoxin
PPHA_RS04450	937799..938107	-	309	WP_012507681.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPHA_RS04455	938247..938879	-	633	WP_012507682.1	DUF4194 domain-containing protein	-
PPHA_RS04460	938879..940330	-	1452	WP_012507683.1	DUF3375 domain-containing protein	-
PPHA_RS04465	940745..941002	+	258	WP_012507684.1	DUF4160 domain-containing protein	-
PPHA_RS04470	941009..941269	+	261	WP_012507685.1	DUF2442 domain-containing protein	-
PPHA_RS04475	941357..942643	-	1287	WP_012507686.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11462.18 Da
• Isoelectric Point: 9.4793

>T22980 WP_012507681.1 NC_011060:c938107-937799 [Pelodictyon phaeoclathratiforme BU-1]
MIEVQQTDVFSKWLSALQDRTAKARILVRIDRLGLGLTGDTKPVGGSVSELRIDYGPGYRLYYTWRGRELVLLLAGGDKS
TQTRDIQKARLLLANLETDEIP

Nucleotide

• Length: 309 bp

>T22980 NC_011060:c938107-937799 [Pelodictyon phaeoclathratiforme BU-1]
ATGATAGAAGTACAACAAACAGACGTTTTTTCAAAATGGTTGTCGGCACTTCAGGATCGTACGGCAAAAGCTCGTATCCT
CGTTCGAATCGACAGGCTCGGGTTGGGTTTAACCGGAGACACCAAACCTGTAGGAGGCTCCGTTTCAGAACTCAGAATCG
ATTATGGCCCAGGTTATCGGCTTTACTATACCTGGCGCGGGCGTGAACTCGTTTTGCTCTTAGCAGGTGGCGATAAAAGT
ACACAGACACGCGATATTCAGAAAGCCAGATTACTTCTTGCAAATCTTGAAACAGATGAGATCCCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10312.85 Da
• Isoelectric Point: 5.8735

>AT22980 WP_012507680.1 NC_011060:c937802-937512 [Pelodictyon phaeoclathratiforme BU-1]
MKTKTRSYDAAEYLATEEEMAAYLEAALEEGEPALVVQALGTIAKARGMSQIARETGLGRESLYKALSLGGNPEFATVIK
VIRALGIRLHAETATK

Nucleotide

• Length: 291 bp

>AT22980 NC_011060:c937802-937512 [Pelodictyon phaeoclathratiforme BU-1]
ATGAAAACAAAAACACGATCCTATGATGCTGCGGAATATCTTGCCACTGAGGAGGAGATGGCGGCATATCTTGAAGCCGC
ACTTGAAGAGGGTGAGCCCGCGCTTGTTGTTCAAGCCTTGGGGACAATCGCTAAAGCAAGAGGGATGTCTCAAATTGCTC
GTGAAACAGGCCTTGGTCGCGAAAGTTTATACAAGGCACTTTCTCTGGGAGGGAATCCTGAATTTGCAACTGTTATTAAA
GTAATTCGGGCATTAGGTATCCGTTTACACGCCGAAACCGCCACCAAGTAA

Structures

Toxin

• AlphaFold DB: B4SF39

Antitoxin

• AlphaFold DB: B4SF38