Detailed information of TA system TA229795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1234182..1234960
• Replicon: chromosome
• Accession: NZ_CP089952
• Organism: Xanthomonas campestris pv. campestris strain SB80

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: LR085_RS05240
• Protein ID: WP_011038220.1
• Coordinates: 1234182..1234673 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: LR085_RS05245
• Protein ID: WP_011038219.1
• Coordinates: 1234670..1234960 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LR085_RS05220 (LR085_05220)	1230433..1231308	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
LR085_RS05225 (LR085_05225)	1231531..1232004	+	474	WP_012437597.1	hypothetical protein	-
LR085_RS05230 (LR085_05230)	1232035..1232712	+	678	WP_003490678.1	response regulator transcription factor	-
LR085_RS05235 (LR085_05235)	1232705..1234039	+	1335	WP_012437598.1	HAMP domain-containing sensor histidine kinase	-
LR085_RS05240 (LR085_05240)	1234182..1234673	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
LR085_RS05245 (LR085_05245)	1234670..1234960	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
LR085_RS05250 (LR085_05250)	1235035..1235436	-	402	WP_019237348.1	SymE family type I addiction module toxin	-
LR085_RS05255 (LR085_05255)	1235968..1236591	-	624	WP_011038217.1	dephospho-CoA kinase	-
LR085_RS05260 (LR085_05260)	1236605..1237468	-	864	WP_011038216.1	A24 family peptidase	-
LR085_RS05265 (LR085_05265)	1237475..1238731	-	1257	WP_011038215.1	type II secretion system F family protein	-
LR085_RS05270 (LR085_05270)	1239058..1239498	+	441	WP_011038214.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1192391..1237759	45368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T229795 WP_011038220.1 NZ_CP089952:c1234673-1234182 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T229795 NZ_CP124517:4020337-4020624 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT229795 WP_011038219.1 NZ_CP089952:c1234960-1234670 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT229795 NZ_CP124517:4020611-4020913 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668