Detailed information of TA system TA229784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 16915..17450
• Replicon: plasmid pETR001-a
• Accession: NZ_CP089943
• Organism: Erwinia tracheiphila strain MDCuke

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LU604_RS25930
• Protein ID: WP_233480948.1
• Coordinates: 17163..17450 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LU604_RS25925
• Protein ID: WP_233480947.1
• Coordinates: 16915..17166 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU604_RS25910 (LU604_25910)	12924..14120	+	1197	WP_233480944.1	conjugal transfer protein TraW	-
LU604_RS25915 (LU604_25915)	14117..14629	+	513	WP_233480945.1	conjugal transfer protein TraX	-
LU604_RS25920 (LU604_25920)	14654..16825	+	2172	WP_233480946.1	DotA/TraY family protein	-
LU604_RS25925 (LU604_25925)	16915..17166	+	252	WP_233480947.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LU604_RS25930 (LU604_25930)	17163..17450	+	288	WP_233480948.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LU604_RS25935 (LU604_25935)	17467..18147	+	681	WP_233480949.1	plasmid IncI1-type surface exclusion protein ExcA	-
LU604_RS25940 (LU604_25940)	18233..19504	+	1272	WP_233480950.1	conjugal transfer protein TrbA	-
LU604_RS25945 (LU604_25945)	19509..20573	+	1065	WP_233480951.1	thioredoxin fold domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71258	71258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11089.10 Da
• Isoelectric Point: 10.7439

>T229784 WP_233480948.1 NZ_CP089943:17163-17450 [Erwinia tracheiphila]
MTYRVKFRTDALKEWNNLDKAIQQQFVRKLKKCAENPHIPSAKLRGMPDCYKIKLQASGFRLVYQVINDELIIAVVAVGK
REHSKVYNLASKRLR

Nucleotide

• Length: 288 bp

>T229784 NZ_CP124517:c2230463-2230056 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9203.44 Da
• Isoelectric Point: 4.5144

>AT229784 WP_233480947.1 NZ_CP089943:16915-17166 [Erwinia tracheiphila]
MPNLIHSDTTASISELKKNPMATVNAGAGSPVAILNHNQPAFYCVPAELYEQMLEYIDEEELAKLVTKRSKQALHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT229784 NZ_CP124517:c2230710-2230444 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA