Detailed information of TA system TA229783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 4701826..4702408
• Replicon: chromosome
• Accession: NZ_CP089942
• Organism: Erwinia tracheiphila strain MDCuke

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0M2KC79
• Locus tag: LU604_RS24860
• Protein ID: WP_016192358.1
• Coordinates: 4701826..4702122 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E8PSG1
• Locus tag: LU604_RS24865
• Protein ID: WP_013583077.1
• Coordinates: 4702124..4702408 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU604_RS24830 (LU604_24830)	4697611..4698201	-	591	WP_016192352.1	type III effector protein	-
LU604_RS24835 (LU604_24835)	4698272..4698493	-	222	WP_233481429.1	hypothetical protein	-
LU604_RS24840 (LU604_24840)	4698706..4699823	+	1118	Protein_4918	RNA-guided endonuclease TnpB family protein	-
LU604_RS24845 (LU604_24845)	4700204..4700494	+	291	WP_233480876.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LU604_RS24850 (LU604_24850)	4700487..4700804	+	318	WP_016192357.1	helix-turn-helix domain-containing protein	-
LU604_RS24855 (LU604_24855)	4700911..4701615	+	705	WP_233479500.1	IS6 family transposase	-
LU604_RS24860 (LU604_24860)	4701826..4702122	+	297	WP_016192358.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LU604_RS24865 (LU604_24865)	4702124..4702408	+	285	WP_013583077.1	putative addiction module antidote protein	Antitoxin
LU604_RS24870 (LU604_24870)	4702860..4703981	+	1122	WP_233481430.1	transposase	-
LU604_RS24875 (LU604_24875)	4704075..4704494	+	420	WP_016192361.1	protein rep	-
LU604_RS24880 (LU604_24880)	4704564..4705166	+	603	WP_233479502.1	pesticin C-terminus-like muramidase	-
LU604_RS24885 (LU604_24885)	4705169..4705582	+	414	WP_016192363.1	hypothetical protein	-
LU604_RS24890 (LU604_24890)	4706116..4706820	+	705	WP_233478933.1	IS6 family transposase	-
LU604_RS24895 (LU604_24895)	4706921..4707286	-	366	WP_233479503.1	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	4694614..4710284	15670
	-	inside	IScluster/Tn	-	ospG	4696778..4710284	13506

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Uncharacterized protein HI1420 (4-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11248.10 Da
• Isoelectric Point: 10.3896

>T229783 WP_016192358.1 NZ_CP089942:4701826-4702122 [Erwinia tracheiphila]
MIEIKRTPEVEKWLKSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRVHQGKGYRVYFGNKNNKIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Nucleotide

• Length: 297 bp

>T229783 NZ_CP124517:c1289096-1288845 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10576.01 Da
• Isoelectric Point: 4.7254

>AT229783 WP_013583077.1 NZ_CP089942:4702124-4702408 [Erwinia tracheiphila]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNMSQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Nucleotide

• Length: 285 bp

>AT229783 NZ_CP124517:c1288848-1288594 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0M2KC79

Antitoxin

• AlphaFold DB: A0A0M2KB14