Detailed information of TA system TA229782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 4700204..4700804
• Replicon: chromosome
• Accession: NZ_CP089942
• Organism: Erwinia tracheiphila strain MDCuke

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LU604_RS24845
• Protein ID: WP_233480876.1
• Coordinates: 4700204..4700494 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LU604_RS24850
• Protein ID: WP_016192357.1
• Coordinates: 4700487..4700804 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU604_RS24825 (LU604_24825)	4696778..4697482	-	705	WP_233479499.1	IS6 family transposase	-
LU604_RS24830 (LU604_24830)	4697611..4698201	-	591	WP_016192352.1	type III effector protein	-
LU604_RS24835 (LU604_24835)	4698272..4698493	-	222	WP_233481429.1	hypothetical protein	-
LU604_RS24840 (LU604_24840)	4698706..4699823	+	1118	Protein_4918	RNA-guided endonuclease TnpB family protein	-
LU604_RS24845 (LU604_24845)	4700204..4700494	+	291	WP_233480876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LU604_RS24850 (LU604_24850)	4700487..4700804	+	318	WP_016192357.1	helix-turn-helix domain-containing protein	Antitoxin
LU604_RS24855 (LU604_24855)	4700911..4701615	+	705	WP_233479500.1	IS6 family transposase	-
LU604_RS24860 (LU604_24860)	4701826..4702122	+	297	WP_016192358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LU604_RS24865 (LU604_24865)	4702124..4702408	+	285	WP_013583077.1	putative addiction module antidote protein	-
LU604_RS24870 (LU604_24870)	4702860..4703981	+	1122	WP_233481430.1	transposase	-
LU604_RS24875 (LU604_24875)	4704075..4704494	+	420	WP_016192361.1	protein rep	-
LU604_RS24880 (LU604_24880)	4704564..4705166	+	603	WP_233479502.1	pesticin C-terminus-like muramidase	-
LU604_RS24885 (LU604_24885)	4705169..4705582	+	414	WP_016192363.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	4694614..4710284	15670
	-	inside	IScluster/Tn	-	ospG	4696778..4710284	13506

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (6-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10669.20 Da
• Isoelectric Point: 4.6484

>T229782 WP_233480876.1 NZ_CP089942:4700204-4700494 [Erwinia tracheiphila]
MPGDPLGKFTFKKRLGLPGVGARDGARSIVFFNDGDNIFFFDIYLKSGLSKKKGKELEDDEIDAYCNIAKDFISMSPATI
NKLLSDKELIEVNCDD

Nucleotide

• Length: 291 bp

>T229782 NZ_CP124517:c1288848-1288594 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11590.53 Da
• Isoelectric Point: 10.8279

>AT229782 WP_016192357.1 NZ_CP089942:4700487-4700804 [Erwinia tracheiphila]
MTERLKRIQGMAERYNKLGVVKDETVAAIDAAVAARNIPAIRVMTGSQIKEVRTRYNMSQAALAITTGMSVESVSKWERN
ESKPNKAALRIINIIDAKGPQVFMA

Nucleotide

• Length: 318 bp

>AT229782 NZ_CP124517:c1289096-1288845 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA