Detailed information of TA system TA229781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3506088..3506712
• Replicon: chromosome
• Accession: NZ_CP089942
• Organism: Erwinia tracheiphila strain MDCuke

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A0M2K484
• Locus tag: LU604_RS18710
• Protein ID: WP_016191644.1
• Coordinates: 3506494..3506712 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LU604_RS18705
• Protein ID: WP_233479014.1
• Coordinates: 3506088..3506468 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU604_RS18685 (LU604_18685)	3501481..3502520	+	1040	Protein_3704	IS481 family transposase	-
LU604_RS18690 (LU604_18690)	3502763..3503641	-	879	WP_016191640.1	metal ABC transporter substrate-binding protein	-
LU604_RS18695 (LU604_18695)	3503638..3504504	-	867	WP_040465679.1	metal ABC transporter permease	-
LU604_RS18700 (LU604_18700)	3504507..3505193	-	687	WP_233479013.1	ABC transporter ATP-binding protein	-
LU604_RS18705 (LU604_18705)	3506088..3506468	+	381	WP_233479014.1	Hha toxicity modulator TomB	Antitoxin
LU604_RS18710 (LU604_18710)	3506494..3506712	+	219	WP_016191644.1	HHA domain-containing protein	Toxin
LU604_RS18720 (LU604_18720)	3507067..3507414	+	348	WP_016191645.1	MGMT family protein	-
LU604_RS18725 (LU604_18725)	3507374..3507925	-	552	Protein_3711	YbaY family lipoprotein	-
LU604_RS18730 (LU604_18730)	3508186..3509025	+	840	Protein_3712	thioesterase family protein	-
LU604_RS18735 (LU604_18735)	3509087..3510391	-	1305	WP_233481368.1	ammonium transporter AmtB	-
LU604_RS18740 (LU604_18740)	3510407..3510745	-	339	WP_016191649.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.08 Da
• Isoelectric Point: 9.7942

>T229781 WP_016191644.1 NZ_CP089942:3506494-3506712 [Erwinia tracheiphila]
MTNKVLTKTDYLMRLRKCRSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKVPVSVWKFIR

Nucleotide

• Length: 219 bp

>T229781 NZ_CP124517:1277687-1278061 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14538.11 Da
• Isoelectric Point: 4.3117

>AT229781 WP_233479014.1 NZ_CP089942:3506088-3506468 [Erwinia tracheiphila]
MDEYSPKRHDVAQLKFLCENLYDESMTTIGDSHHGWINDPTSASNLQLNDLIEHIASFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGINVQELQRWRKSAEGLFSIFAEECAQVANPASHSF

Nucleotide

• Length: 381 bp

>AT229781 NZ_CP124517:1277230-1277598 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0M2K484