Detailed information of TA system TA022978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 258930..259512
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4SBS6
• Locus tag: PPHA_RS01345
• Protein ID: WP_012507097.1
• Coordinates: 259177..259512 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4SBS5
• Locus tag: PPHA_RS01340
• Protein ID: WP_012507096.1
• Coordinates: 258930..259172 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS01320	256608..257081	-	474	Protein_243	HipA domain-containing protein	-
PPHA_RS01325	257063..257392	-	330	WP_012507094.1	helix-turn-helix domain-containing protein	-
PPHA_RS01335	257656..258849	+	1194	WP_041526377.1	ATP-binding protein	-
PPHA_RS01340	258930..259172	+	243	WP_012507096.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PPHA_RS01345	259177..259512	+	336	WP_012507097.1	endoribonuclease MazF	Toxin
PPHA_RS01350	259628..260872	-	1245	WP_012507098.1	type II toxin-antitoxin system HipA family toxin	-
PPHA_RS01355	260859..261158	-	300	WP_012507099.1	helix-turn-helix domain-containing protein	-
PPHA_RS01360	261414..261617	+	204	WP_150085546.1	hypothetical protein	-
PPHA_RS01365	262356..262943	+	588	WP_012507100.1	toll/interleukin-1 receptor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-108)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12174.26 Da
• Isoelectric Point: 7.2419

>T22978 WP_012507097.1 NC_011060:259177-259512 [Pelodictyon phaeoclathratiforme BU-1]
MAYTPDRGDMVWIAFNPQAGHEQAGRRPALVLSPKAYNSKVGLALLCPITSQIKGYPFEVLLPEGLNINGAILSDQVKSL
DWKIRQAELICLLPSSVVDEVLHKLHTLLGL

Nucleotide

• Length: 336 bp

>T22978 NC_011060:259177-259512 [Pelodictyon phaeoclathratiforme BU-1]
ATGGCTTATACTCCGGATCGTGGTGACATGGTGTGGATTGCCTTTAATCCTCAGGCGGGTCATGAGCAGGCAGGACGTCG
TCCAGCATTGGTCTTGTCTCCCAAAGCGTACAACTCCAAAGTTGGTCTGGCGCTTCTTTGCCCCATCACCAGCCAGATTA
AAGGGTATCCCTTTGAAGTGCTGCTTCCTGAAGGGTTGAACATCAATGGCGCGATTTTATCTGATCAGGTAAAAAGTCTT
GACTGGAAGATCCGGCAGGCAGAGTTAATTTGCCTATTGCCCTCGTCGGTAGTTGATGAAGTGCTGCATAAGTTGCATAC
CCTGTTGGGGTTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.18 Da
• Isoelectric Point: 6.4563

>AT22978 WP_012507096.1 NC_011060:258930-259172 [Pelodictyon phaeoclathratiforme BU-1]
MLTKVQKWGNSLALRIPKTWALEARLENNSEVEISLVEGKLVITPVVTIKQDLAHLLSGVTKENLHNECDTGNAAGKENW

Nucleotide

• Length: 243 bp

>AT22978 NC_011060:258930-259172 [Pelodictyon phaeoclathratiforme BU-1]
ATGCTCACAAAGGTTCAGAAGTGGGGCAACAGCCTTGCCTTGAGAATACCGAAAACCTGGGCGCTTGAGGCCAGGCTCGA
AAACAATTCAGAGGTAGAGATTTCTCTGGTTGAGGGAAAACTCGTTATCACTCCAGTGGTCACTATAAAACAAGATCTTG
CTCACCTGCTTTCCGGCGTCACGAAAGAAAATCTGCATAACGAATGCGATACCGGTAATGCTGCAGGCAAAGAAAACTGG
TAA

Structures

Toxin

• AlphaFold DB: B4SBS6

Antitoxin

• AlphaFold DB: B4SBS5