Detailed information of TA system TA229779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3262357..3262953
• Replicon: chromosome
• Accession: NZ_CP089942
• Organism: Erwinia tracheiphila strain MDCuke

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A822X842
• Locus tag: LU604_RS17460
• Protein ID: WP_063162134.1
• Coordinates: 3262357..3262536 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LU604_RS17465
• Protein ID: WP_233478881.1
• Coordinates: 3262558..3262953 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU604_RS17420 (LU604_17420)	3257905..3259025	+	1121	WP_233478879.1	IS3 family transposase	-
LU604_RS17425 (LU604_17425)	3259066..3259200	-	135	Protein_3455	helix-turn-helix domain-containing protein	-
LU604_RS17430 (LU604_17430)	3259371..3259811	-	441	Protein_3456	IS200/IS605 family transposase	-
LU604_RS17435 (LU604_17435)	3259955..3260242	-	288	WP_016191801.1	RnfH family protein	-
LU604_RS17440 (LU604_17440)	3260235..3260669	-	435	WP_016191800.1	type II toxin-antitoxin system RatA family toxin	-
LU604_RS17445 (LU604_17445)	3260828..3261310	+	483	WP_016191799.1	SsrA-binding protein SmpB	-
LU604_RS17455 (LU604_17455)	3262010..3262255	+	246	WP_233478880.1	DinI-like family protein	-
LU604_RS17460 (LU604_17460)	3262357..3262536	+	180	WP_063162134.1	type II toxin-antitoxin system HicA family toxin	Toxin
LU604_RS17465 (LU604_17465)	3262558..3262953	+	396	WP_233478881.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LU604_RS17470 (LU604_17470)	3263004..3264044	-	1041	WP_233478553.1	IS481 family transposase	-
LU604_RS17475 (LU604_17475)	3264208..3264567	+	360	WP_233478883.1	GtrA family protein	-
LU604_RS17480 (LU604_17480)	3264564..3265484	+	921	WP_233478884.1	glycosyltransferase family 2 protein	-
LU604_RS17485 (LU604_17485)	3265484..3266701	+	1218	WP_233478885.1	DUF6056 family protein	-
LU604_RS17490 (LU604_17490)	3266749..3267180	-	432	WP_233478886.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3257905..3264044	6139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6674.94 Da
• Isoelectric Point: 10.9132

>T229779 WP_063162134.1 NZ_CP089942:3262357-3262536 [Erwinia tracheiphila]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPMKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T229779 NZ_CP124517:c735787-734465 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14324.14 Da
• Isoelectric Point: 4.1604

>AT229779 WP_233478881.1 NZ_CP089942:3262558-3262953 [Erwinia tracheiphila]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESVREAIDAHIELLVEDGESVPEATNVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVY

Nucleotide

• Length: 396 bp

>AT229779 NZ_CP124517:c736053-735787 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A822X842