Detailed information of TA system TA229769

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1136236..1136884
• Replicon: chromosome
• Accession: NZ_CP089942
• Organism: Erwinia tracheiphila strain MDCuke

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A0M2KDQ7
• Locus tag: LU604_RS05980
• Protein ID: WP_016190246.1
• Coordinates: 1136483..1136884 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A0M2KLB4
• Locus tag: LU604_RS05975
• Protein ID: WP_016190247.1
• Coordinates: 1136236..1136502 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU604_RS05945 (LU604_05945)	1131610..1133049	-	1440	WP_233480154.1	6-phospho-beta-glucosidase	-
LU604_RS05950 (LU604_05950)	1133243..1133713	-	471	Protein_1178	PTS transporter subunit EIIB	-
LU604_RS05955 (LU604_05955)	1133728..1134118	+	391	Protein_1179	hemolysin III family protein	-
LU604_RS05960 (LU604_05960)	1134168..1134865	+	698	WP_233480155.1	IS1 family transposase	-
LU604_RS05965 (LU604_05965)	1134887..1134997	+	111	Protein_1181	hemolysin III family protein	-
LU604_RS05970 (LU604_05970)	1135039..1136019	-	981	WP_233480156.1	tRNA-modifying protein YgfZ	-
LU604_RS05975 (LU604_05975)	1136236..1136502	+	267	WP_016190247.1	FAD assembly factor SdhE	Antitoxin
LU604_RS05980 (LU604_05980)	1136483..1136884	+	402	WP_016190246.1	protein YgfX	Toxin
LU604_RS05985 (LU604_05985)	1136994..1137512	-	519	WP_233480157.1	flavodoxin FldB	-
LU604_RS05990 (LU604_05990)	1137611..1138504	+	894	WP_016190244.1	site-specific tyrosine recombinase XerD	-
LU604_RS05995 (LU604_05995)	1138548..1139252	+	705	WP_016190243.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LU604_RS06000 (LU604_06000)	1139258..1140976	+	1719	WP_233480158.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hsiB1/vipA / hsiC1/vipB	746383..1192486	446103
	-	flank	IS/Tn	-	-	1134362..1134865	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-130)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15786.53 Da
• Isoelectric Point: 12.0140

>T229769 WP_016190246.1 NZ_CP089942:1136483-1136884 [Erwinia tracheiphila]
VALWHSELRVSWRAQWFSLLLHGVAILIVLLAPWPASYTVVWMSLLTLTIFECVRSQRRIGNREGEIALFEGRQVQWRQK
RWQIRGRPWITRQAILMTLRSATGEREKLWLFSDSMSAEDWRSLRKQLLRAAL

Nucleotide

• Length: 402 bp

>T229769 NZ_CP124515:c3602290-3601988 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10492.04 Da
• Isoelectric Point: 4.8102

>AT229769 WP_016190247.1 NZ_CP089942:1136236-1136502 [Erwinia tracheiphila]
MDISNKARVHWACRRGMRELDISIMPFFEYEYDSLSDSDKLVFVRLLENDDPDLFNWLMNYGEPPDTELKRMVLLIQQRN
KQRGPVAL

Nucleotide

• Length: 267 bp

>AT229769 NZ_CP124515:c3601952-3601611 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0M2KDQ7

Antitoxin

• AlphaFold DB: A0A0M2KLB4