229764

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 4860081..4860663 Replicon chromosome
Accession NZ_CP089940
Organism Erwinia tracheiphila strain BuffGH

Toxin (Protein)


Gene name PumA Uniprot ID A0A0M2KC79
Locus tag LU631_RS25280 Protein ID WP_016192358.1
Coordinates 4860081..4860377 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID E8PSG1
Locus tag LU631_RS25285 Protein ID WP_013583077.1
Coordinates 4860379..4860663 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU631_RS25250 (LU631_25250) 4855865..4856455 - 591 WP_016192352.1 type III effector protein -
LU631_RS25255 (LU631_25255) 4856526..4856783 - 258 WP_016192353.1 hypothetical protein -
LU631_RS25260 (LU631_25260) 4856960..4858077 + 1118 Protein_4990 RNA-guided endonuclease TnpB family protein -
LU631_RS25265 (LU631_25265) 4858458..4858748 + 291 WP_232426910.1 type II toxin-antitoxin system RelE/ParE family toxin -
LU631_RS25270 (LU631_25270) 4858741..4859058 + 318 WP_016192357.1 helix-turn-helix domain-containing protein -
LU631_RS25275 (LU631_25275) 4859165..4859870 + 706 Protein_4993 IS6 family transposase -
LU631_RS25280 (LU631_25280) 4860081..4860377 + 297 WP_016192358.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LU631_RS25285 (LU631_25285) 4860379..4860663 + 285 WP_013583077.1 putative addiction module antidote protein Antitoxin
LU631_RS25290 (LU631_25290) 4860963..4862003 + 1041 WP_046371813.1 IS481 family transposase -
LU631_RS25295 (LU631_25295) 4862323..4863440 + 1118 Protein_4997 RNA-guided endonuclease TnpB family protein -
LU631_RS25300 (LU631_25300) 4863534..4863953 + 420 WP_016192361.1 protein rep -
LU631_RS25305 (LU631_25305) 4864023..4864631 + 609 WP_016192362.1 pesticin C-terminus-like muramidase -
LU631_RS25310 (LU631_25310) 4864628..4865041 + 414 WP_016192363.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - ospG 4834643..4912538 77895
- inside IScluster/Tn - ospG 4855032..4889280 34248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Uncharacterized protein HI1420

(4-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11248.10 Da        Isoelectric Point: 10.3896

>T229764 WP_016192358.1 NZ_CP089940:4860081-4860377 [Erwinia tracheiphila]
MIEIKRTPEVEKWLKSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRVHQGKGYRVYFGNKNNKIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Download         Length: 297 bp

>T229764 NZ_CP124515:c2023639-2023265 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10576.01 Da        Isoelectric Point: 4.7254

>AT229764 WP_013583077.1 NZ_CP089940:4860379-4860663 [Erwinia tracheiphila]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNMSQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Download         Length: 285 bp

>AT229764 NZ_CP124515:c2024096-2023728 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2KC79

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2KB14

References