Detailed information of TA system TA229762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3950581..3951182
• Replicon: chromosome
• Accession: NZ_CP089940
• Organism: Erwinia tracheiphila strain BuffGH

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0M2K9A2
• Locus tag: LU631_RS20710
• Protein ID: WP_046371846.1
• Coordinates: 3950581..3950964 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: H3R939
• Locus tag: LU631_RS20715
• Protein ID: WP_006117881.1
• Coordinates: 3950961..3951182 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU631_RS20685 (LU631_20685)	3945633..3945773	+	141	WP_161796965.1	hypothetical protein	-
LU631_RS20690 (LU631_20690)	3945782..3946996	+	1215	WP_046371879.1	IS91 family transposase	-
LU631_RS20695 (LU631_20695)	3947148..3948539	+	1392	Protein_4093	terminase family protein	-
LU631_RS20700 (LU631_20700)	3948539..3949597	+	1059	WP_046371980.1	phage portal protein	-
LU631_RS20705 (LU631_20705)	3949668..3950171	-	504	WP_016193219.1	hypothetical protein	-
LU631_RS20710 (LU631_20710)	3950581..3950964	-	384	WP_046371846.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LU631_RS20715 (LU631_20715)	3950961..3951182	-	222	WP_006117881.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LU631_RS20720 (LU631_20720)	3951249..3951944	-	696	WP_046371847.1	DNA methylase	-
LU631_RS20725 (LU631_20725)	3952194..3952553	-	360	WP_046371849.1	type II toxin-antitoxin system HicB family antitoxin	-
LU631_RS20730 (LU631_20730)	3952573..3952839	-	267	WP_046371850.1	hypothetical protein	-
LU631_RS20735 (LU631_20735)	3952805..3955300	-	2496	WP_052734703.1	replication endonuclease	-
LU631_RS20740 (LU631_20740)	3955300..3955683	-	384	WP_046371981.1	hypothetical protein	-
LU631_RS20745 (LU631_20745)	3955756..3955998	-	243	WP_016193223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3929205..4005344	76139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-59)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14129.14 Da
• Isoelectric Point: 4.9094

>T229762 WP_046371846.1 NZ_CP089940:c3950964-3950581 [Erwinia tracheiphila]
MKWISAQEVIAFHDRLLTALPGVQGMPEPGRAEAIIYRVQNQLYYEGVEDIHELAAMYLVAISRGHIFNDGNKRTAFFVA
MAFLKRNGIDIRDEGDELEELTVSAAMGAVSSDDIAAVLRRLSINPH

Nucleotide

• Length: 384 bp

>T229762 NZ_CP124515:2010355-2010606 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8110.06 Da
• Isoelectric Point: 5.0644

>AT229762 WP_006117881.1 NZ_CP089940:c3951182-3950961 [Erwinia tracheiphila]
MRTYTTSQARQNIAEVMEAATAGEPVEITRRDGSAAVLISRDDFNAWQEAKLDAEFAEIMGRHGRTIKALADR

Nucleotide

• Length: 222 bp

>AT229762 NZ_CP124515:2010603-2010857 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0M2K9A2

Antitoxin

• AlphaFold DB: A0A068Z6U5