Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3876787..3877435 Replicon chromosome
Accession NZ_CP089940
Organism Erwinia tracheiphila strain BuffGH

Toxin (Protein)


Gene name cptA Uniprot ID A0A0M2KDQ7
Locus tag LU631_RS20325 Protein ID WP_016190246.1
Coordinates 3876787..3877188 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0M2KLB4
Locus tag LU631_RS20330 Protein ID WP_016190247.1
Coordinates 3877169..3877435 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU631_RS20305 (LU631_20305) 3872695..3874413 - 1719 WP_016190242.1 single-stranded-DNA-specific exonuclease RecJ -
LU631_RS20310 (LU631_20310) 3874419..3875123 - 705 WP_016190243.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LU631_RS20315 (LU631_20315) 3875167..3876060 - 894 WP_016190244.1 site-specific tyrosine recombinase XerD -
LU631_RS20320 (LU631_20320) 3876159..3876677 + 519 WP_016190245.1 flavodoxin FldB -
LU631_RS20325 (LU631_20325) 3876787..3877188 - 402 WP_016190246.1 protein YgfX Toxin
LU631_RS20330 (LU631_20330) 3877169..3877435 - 267 WP_016190247.1 FAD assembly factor SdhE Antitoxin
LU631_RS20335 (LU631_20335) 3877652..3878632 + 981 WP_016190248.1 tRNA-modifying protein YgfZ -
LU631_RS20340 (LU631_20340) 3878674..3878784 - 111 Protein_4024 hemolysin III family protein -
LU631_RS20345 (LU631_20345) 3878806..3879309 - 504 WP_046371978.1 IS1 family transposase -
LU631_RS20355 (LU631_20355) 3879553..3879943 - 391 Protein_4026 hemolysin III family protein -
LU631_RS20360 (LU631_20360) 3879958..3880428 + 471 Protein_4027 PTS transporter subunit EIIB -
LU631_RS20365 (LU631_20365) 3880622..3882061 + 1440 WP_016190252.1 6-phospho-beta-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3878806..3879309 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(2-130)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 15786.53 Da        Isoelectric Point: 12.0140

>T229761 WP_016190246.1 NZ_CP089940:c3877188-3876787 [Erwinia tracheiphila]
VALWHSELRVSWRAQWFSLLLHGVAILIVLLAPWPASYTVVWMSLLTLTIFECVRSQRRIGNREGEIALFEGRQVQWRQK
RWQIRGRPWITRQAILMTLRSATGEREKLWLFSDSMSAEDWRSLRKQLLRAAL

Download         Length: 402 bp

>T229761 NZ_CP124515:976016-976423 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10492.04 Da        Isoelectric Point: 4.8102

>AT229761 WP_016190247.1 NZ_CP089940:c3877435-3877169 [Erwinia tracheiphila]
MDISNKARVHWACRRGMRELDISIMPFFEYEYDSLSDSDKLVFVRLLENDDPDLFNWLMNYGEPPDTELKRMVLLIQQRN
KQRGPVAL

Download         Length: 267 bp

>AT229761 NZ_CP124515:975769-976035 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2KDQ7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2KLB4

References