Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1557596..1558219 Replicon chromosome
Accession NZ_CP089940
Organism Erwinia tracheiphila strain BuffGH

Toxin (Protein)


Gene name Hha Uniprot ID A0A0M2K484
Locus tag LU631_RS08215 Protein ID WP_016191644.1
Coordinates 1557596..1557814 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0M2KB13
Locus tag LU631_RS08220 Protein ID WP_016191643.1
Coordinates 1557839..1558219 (-) Length 127 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU631_RS08185 (LU631_08185) 1553563..1553901 + 339 WP_016191649.1 P-II family nitrogen regulator -
LU631_RS08190 (LU631_08190) 1553917..1555221 + 1305 WP_087949710.1 ammonium transporter AmtB -
LU631_RS08195 (LU631_08195) 1555283..1556122 - 840 Protein_1618 thioesterase family protein -
LU631_RS08200 (LU631_08200) 1556375..1556934 + 560 Protein_1619 YbaY family lipoprotein -
LU631_RS08205 (LU631_08205) 1556894..1557241 - 348 WP_016191645.1 MGMT family protein -
LU631_RS08215 (LU631_08215) 1557596..1557814 - 219 WP_016191644.1 HHA domain-containing protein Toxin
LU631_RS08220 (LU631_08220) 1557839..1558219 - 381 WP_016191643.1 Hha toxicity modulator TomB Antitoxin
LU631_RS08225 (LU631_08225) 1559114..1559800 + 687 WP_046372383.1 ABC transporter ATP-binding protein -
LU631_RS08230 (LU631_08230) 1559803..1560669 + 867 WP_040465679.1 metal ABC transporter permease -
LU631_RS08235 (LU631_08235) 1560666..1561544 + 879 WP_016191640.1 metal ABC transporter substrate-binding protein -
LU631_RS08240 (LU631_08240) 1561787..1562826 - 1040 Protein_1626 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - acrB / acrA 1346249..1667188 320939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.08 Da        Isoelectric Point: 9.7942

>T229748 WP_016191644.1 NZ_CP089940:c1557814-1557596 [Erwinia tracheiphila]
MTNKVLTKTDYLMRLRKCRSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKVPVSVWKFIR

Download         Length: 219 bp

>T229748 NZ_CP124513:c3808825-3807905 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin


Download         Length: 127 a.a.        Molecular weight: 14552.13 Da        Isoelectric Point: 4.3117

>AT229748 WP_016191643.1 NZ_CP089940:c1558219-1557839 [Erwinia tracheiphila]
MDEYSPKRHDIAQLKFLCENLYDESMTTIGDSHHGWINDPTSASNLQLNDLIEHIASFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGINVQELQRWRKSAEGLFSIFAEECAQVANPASHSF

Download         Length: 381 bp

>AT229748 NZ_CP124513:c3809594-3808812 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2K484

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2KB13

References