Detailed information of TA system TA229745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 16916..17451
• Replicon: plasmid pETR001-b
• Accession: NZ_CP089935
• Organism: Erwinia tracheiphila strain SCR3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LU632_RS25410
• Protein ID: WP_233480948.1
• Coordinates: 17164..17451 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LU632_RS25405
• Protein ID: WP_233480947.1
• Coordinates: 16916..17167 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU632_RS25390 (LU632_25390)	12925..14121	+	1197	WP_233480944.1	conjugal transfer protein TraW	-
LU632_RS25395 (LU632_25395)	14118..14630	+	513	WP_233480945.1	conjugal transfer protein TraX	-
LU632_RS25400 (LU632_25400)	14655..16826	+	2172	WP_233480946.1	DotA/TraY family protein	-
LU632_RS25405 (LU632_25405)	16916..17167	+	252	WP_233480947.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LU632_RS25410 (LU632_25410)	17164..17451	+	288	WP_233480948.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LU632_RS25415 (LU632_25415)	17468..18148	+	681	WP_233480949.1	plasmid IncI1-type surface exclusion protein ExcA	-
LU632_RS25420 (LU632_25420)	18234..19505	+	1272	WP_233480950.1	conjugal transfer protein TrbA	-
LU632_RS25425 (LU632_25425)	19510..20574	+	1065	WP_233480951.1	thioredoxin fold domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..68396	68396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11089.10 Da
• Isoelectric Point: 10.7439

>T229745 WP_233480948.1 NZ_CP089935:17164-17451 [Erwinia tracheiphila]
MTYRVKFRTDALKEWNNLDKAIQQQFVRKLKKCAENPHIPSAKLRGMPDCYKIKLQASGFRLVYQVINDELIIAVVAVGK
REHSKVYNLASKRLR

Nucleotide

• Length: 288 bp

>T229745 NZ_CP124513:2956784-2957062 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9203.44 Da
• Isoelectric Point: 4.5144

>AT229745 WP_233480947.1 NZ_CP089935:16916-17167 [Erwinia tracheiphila]
MPNLIHSDTTASISELKKNPMATVNAGAGSPVAILNHNQPAFYCVPAELYEQMLEYIDEEELAKLVTKRSKQALHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT229745 NZ_CP124513:2956591-2956782 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA