Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 4641027..4641609 Replicon chromosome
Accession NZ_CP089934
Organism Erwinia tracheiphila strain SCR3

Toxin (Protein)


Gene name PumA Uniprot ID A0A0M2KC79
Locus tag LU632_RS24320 Protein ID WP_016192358.1
Coordinates 4641027..4641323 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID E8PSG1
Locus tag LU632_RS24325 Protein ID WP_013583077.1
Coordinates 4641325..4641609 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU632_RS24290 (LU632_24290) 4636812..4637402 - 591 WP_016192352.1 type III effector protein -
LU632_RS24295 (LU632_24295) 4637473..4637730 - 258 WP_016192353.1 hypothetical protein -
LU632_RS24300 (LU632_24300) 4637907..4639024 + 1118 Protein_4804 RNA-guided endonuclease TnpB family protein -
LU632_RS24305 (LU632_24305) 4639405..4639695 + 291 WP_233480876.1 type II toxin-antitoxin system RelE/ParE family toxin -
LU632_RS24310 (LU632_24310) 4639688..4640005 + 318 WP_016192357.1 helix-turn-helix domain-containing protein -
LU632_RS24315 (LU632_24315) 4640112..4640816 + 705 WP_233479500.1 IS6 family transposase -
LU632_RS24320 (LU632_24320) 4641027..4641323 + 297 WP_016192358.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LU632_RS24325 (LU632_24325) 4641325..4641609 + 285 WP_013583077.1 putative addiction module antidote protein Antitoxin
LU632_RS24330 (LU632_24330) 4642061..4643182 + 1122 WP_233479501.1 transposase -
LU632_RS24335 (LU632_24335) 4643276..4643695 + 420 WP_016192361.1 protein rep -
LU632_RS24340 (LU632_24340) 4643765..4644367 + 603 WP_233479502.1 pesticin C-terminus-like muramidase -
LU632_RS24345 (LU632_24345) 4644370..4644783 + 414 WP_016192363.1 hypothetical protein -
LU632_RS24350 (LU632_24350) 4645318..4646022 + 705 WP_233478933.1 IS6 family transposase -
LU632_RS24355 (LU632_24355) 4646123..4646488 - 366 WP_233479503.1 type II toxin-antitoxin system PemK/MazF family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ospG 4633815..4649486 15671
- inside IScluster/Tn - ospG 4635979..4649486 13507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Uncharacterized protein HI1420

(4-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11248.10 Da        Isoelectric Point: 10.3896

>T229744 WP_016192358.1 NZ_CP089934:4641027-4641323 [Erwinia tracheiphila]
MIEIKRTPEVEKWLKSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRVHQGKGYRVYFGNKNNKIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Download         Length: 297 bp

>T229744 NZ_CP124513:2793249-2793356 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10576.01 Da        Isoelectric Point: 4.7254

>AT229744 WP_013583077.1 NZ_CP089934:4641325-4641609 [Erwinia tracheiphila]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNMSQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Download         Length: 285 bp

>AT229744 NZ_CP124513:c2793192-2793137 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2KC79

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2KB14

References