Detailed information of TA system TA229740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3210514..3211110
• Replicon: chromosome
• Accession: NZ_CP089934
• Organism: Erwinia tracheiphila strain SCR3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A822X842
• Locus tag: LU632_RS17040
• Protein ID: WP_063162134.1
• Coordinates: 3210514..3210693 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LU632_RS17045
• Protein ID: WP_233478881.1
• Coordinates: 3210715..3211110 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU632_RS17000 (LU632_17000)	3206062..3207182	+	1121	WP_233478879.1	IS3 family transposase	-
LU632_RS17005 (LU632_17005)	3207223..3207357	-	135	Protein_3367	helix-turn-helix domain-containing protein	-
LU632_RS17010 (LU632_17010)	3207528..3207968	-	441	Protein_3368	IS200/IS605 family transposase	-
LU632_RS17015 (LU632_17015)	3208112..3208399	-	288	WP_016191801.1	RnfH family protein	-
LU632_RS17020 (LU632_17020)	3208392..3208826	-	435	WP_016191800.1	type II toxin-antitoxin system RatA family toxin	-
LU632_RS17025 (LU632_17025)	3208985..3209467	+	483	WP_016191799.1	SsrA-binding protein SmpB	-
LU632_RS17035 (LU632_17035)	3210167..3210412	+	246	WP_233478880.1	DinI-like family protein	-
LU632_RS17040 (LU632_17040)	3210514..3210693	+	180	WP_063162134.1	type II toxin-antitoxin system HicA family toxin	Toxin
LU632_RS17045 (LU632_17045)	3210715..3211110	+	396	WP_233478881.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LU632_RS17050 (LU632_17050)	3211161..3212201	-	1041	WP_233478882.1	IS481 family transposase	-
LU632_RS17055 (LU632_17055)	3212365..3212724	+	360	WP_233478883.1	GtrA family protein	-
LU632_RS17060 (LU632_17060)	3212721..3213641	+	921	WP_233478884.1	glycosyltransferase family 2 protein	-
LU632_RS17065 (LU632_17065)	3213641..3214858	+	1218	WP_233478885.1	DUF6056 family protein	-
LU632_RS17070 (LU632_17070)	3214906..3215337	-	432	WP_233478886.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gtrB / gtrB	3199754..3247134	47380
	-	inside	IScluster/Tn	-	-	3206062..3212201	6139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6674.94 Da
• Isoelectric Point: 10.9132

>T229740 WP_063162134.1 NZ_CP089934:3210514-3210693 [Erwinia tracheiphila]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPMKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T229740 NZ_CP124513:2000951-2001205 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14324.14 Da
• Isoelectric Point: 4.1604

>AT229740 WP_233478881.1 NZ_CP089934:3210715-3211110 [Erwinia tracheiphila]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESVREAIDAHIELLVEDGESVPEATNVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVY

Nucleotide

• Length: 396 bp

>AT229740 NZ_CP124513:2000703-2000954 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A822X842