Detailed information of TA system TA022974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1889539..1890332
• Replicon: chromosome
• Accession: NC_011059
• Organism: Prosthecochloris aestuarii DSM 271

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: PAES_RS08645
• Protein ID: WP_012506280.1
• Coordinates: 1889539..1890054 (-)
• Length: 172 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B4S3K9
• Locus tag: PAES_RS08650
• Protein ID: WP_012506281.1
• Coordinates: 1890051..1890332 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAES_RS08630	1884977..1885852	-	876	WP_012506277.1	glucose-1-phosphate thymidylyltransferase RfbA	-
PAES_RS08635	1886034..1887455	-	1422	WP_012506278.1	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	-
PAES_RS08640	1888323..1888805	+	483	WP_012506279.1	hypothetical protein	-
PAES_RS08645	1889539..1890054	-	516	WP_012506280.1	GNAT family N-acetyltransferase	Toxin
PAES_RS08650	1890051..1890332	-	282	WP_012506281.1	DUF1778 domain-containing protein	Antitoxin
PAES_RS08655	1891006..1891758	-	753	WP_012506283.1	YdcF family protein	-
PAES_RS08660	1892273..1893682	-	1410	WP_012506284.1	phosphomannomutase	-
PAES_RS08665	1894141..1895124	+	984	WP_012506285.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1872667..1936156	63489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (11-87)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 18745.57 Da
• Isoelectric Point: 8.9260

>T22974 WP_012506280.1 NC_011059:c1890054-1889539 [Prosthecochloris aestuarii DSM 271]
MSGSGFNQVQKLASSHDVSIFDCGNKALNQFLQRYAFVNQKANSAQTYVCCEQGTHCVAGFYSLTVGSVEPSAAVGRLSK
GLARHPVPVMILARLAVDCRHQGVGLGRALLKDALLRTAQAAELAGIRAILVHAKDDDARNWYLQWGFEQSPTDPYHLFL
LIKDLLAFLKR

Nucleotide

• Length: 516 bp

>T22974 NC_011059:c1890054-1889539 [Prosthecochloris aestuarii DSM 271]
TTGAGTGGTTCGGGTTTTAACCAGGTACAGAAGCTTGCATCCAGCCACGATGTTTCGATATTCGATTGTGGAAATAAGGC
CTTGAATCAGTTCCTGCAACGTTATGCTTTTGTAAACCAGAAGGCGAACAGTGCACAGACCTATGTTTGTTGTGAACAGG
GAACTCATTGCGTTGCGGGTTTTTACAGCCTCACTGTCGGAAGTGTAGAACCGTCAGCGGCAGTTGGTCGACTTAGCAAA
GGCCTTGCCCGGCATCCGGTGCCTGTGATGATTTTGGCGAGGCTTGCGGTTGATTGTCGTCATCAGGGTGTTGGGCTTGG
TAGAGCCTTGCTTAAGGATGCTTTGTTGCGAACAGCCCAGGCTGCTGAACTTGCCGGTATAAGGGCTATTCTTGTCCATG
CGAAGGACGATGATGCCCGTAACTGGTATCTGCAATGGGGATTCGAACAAAGTCCTACCGATCCTTATCACCTTTTTCTT
CTGATCAAAGACCTGCTTGCCTTTCTTAAACGGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10353.74 Da
• Isoelectric Point: 6.2217

>AT22974 WP_012506281.1 NC_011059:c1890332-1890051 [Prosthecochloris aestuarii DSM 271]
MATQATIKTERIDVRANLSVKQLLQEAARACHKSVSEFLLDAGVHAANQALAERRGFELDDARWQEFQELLDRPVSAPHG
RMKSLLSEPGVLD

Nucleotide

• Length: 282 bp

>AT22974 NC_011059:c1890332-1890051 [Prosthecochloris aestuarii DSM 271]
ATGGCTACTCAAGCGACTATCAAAACTGAACGGATCGATGTCCGTGCGAATCTGTCGGTCAAGCAATTGTTGCAAGAGGC
AGCAAGGGCTTGCCATAAAAGTGTCAGCGAATTTCTGTTGGATGCCGGTGTGCATGCAGCTAACCAGGCGCTTGCTGAGC
GCCGAGGGTTTGAACTTGATGATGCCCGGTGGCAGGAGTTTCAAGAGCTCCTTGATAGGCCGGTTTCGGCACCTCATGGC
AGGATGAAATCACTGCTTTCAGAACCGGGGGTACTTGATTGA

Structures

Antitoxin

• AlphaFold DB: B4S3K9