Detailed information of TA system TA022973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1210967..1211652
• Replicon: chromosome
• Accession: NC_011035
• Organism: Neisseria gonorrhoeae NCCP11945

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q5F6D1
• Locus tag: NGK_RS06545
• Protein ID: WP_003689143.1
• Coordinates: 1210967..1211149 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q5F6D2
• Locus tag: NGK_RS06550
• Protein ID: WP_003691454.1
• Coordinates: 1211251..1211652 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK_RS06510	1205973..1206872	-	900	WP_041421306.1	replication protein	-
NGK_RS15435	1207069..1207269	-	201	WP_012503750.1	hypothetical protein	-
NGK_RS06520	1207271..1207456	-	186	WP_048654326.1	helix-turn-helix domain-containing protein	-
NGK_RS12245	1207621..1208307	+	687	WP_012503751.1	helix-turn-helix transcriptional regulator	-
NGK_RS06530	1208347..1209045	+	699	WP_002212401.1	helix-turn-helix domain-containing protein	-
NGK_RS06535	1209284..1209982	+	699	WP_003689139.1	hypothetical protein	-
NGK_RS06540	1209979..1210797	+	819	WP_012503752.1	DUF3037 domain-containing protein	-
NGK_RS06545	1210967..1211149	+	183	WP_003689143.1	type II toxin-antitoxin system HicA family toxin	Toxin
NGK_RS06550	1211251..1211652	+	402	WP_003691454.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NGK_RS06555	1211751..1212533	-	783	WP_003689146.1	hypothetical protein	-
NGK_RS06560	1212733..1212933	+	201	WP_003704298.1	hypothetical protein	-
NGK_RS06565	1212966..1213442	+	477	WP_002255718.1	hypothetical protein	-
NGK_RS06570	1213439..1213726	+	288	WP_041421246.1	hypothetical protein	-
NGK_RS06575	1213867..1214199	+	333	WP_003687946.1	hypothetical protein	-
NGK_RS06580	1214352..1214630	+	279	WP_041421244.1	hypothetical protein	-
NGK_RS06585	1214627..1214788	+	162	WP_003691530.1	hypothetical protein	-
NGK_RS06590	1214857..1215543	+	687	WP_012503754.1	hypothetical protein	-
NGK_RS06595	1215683..1215865	+	183	WP_003691535.1	hypothetical protein	-
NGK_RS06600	1215862..1216353	+	492	WP_003691537.1	siphovirus Gp157 family protein	-
NGK_RS06605	1216405..1216620	+	216	WP_003691538.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1163221..1232748	69527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6687.77 Da
• Isoelectric Point: 10.7235

>T22973 WP_003689143.1 NC_011035:1210967-1211149 [Neisseria gonorrhoeae NCCP11945]
MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKDLPTGTVKNIYKQAGLK

Nucleotide

• Length: 183 bp

>T22973 NC_011035:1210967-1211149 [Neisseria gonorrhoeae NCCP11945]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14634.43 Da
• Isoelectric Point: 4.5457

>AT22973 WP_003691454.1 NC_011035:1211251-1211652 [Neisseria gonorrhoeae NCCP11945]
MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAYMHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVM
IEIDPAKISRQQIRFNVSWPQYLLDRVDEYTSANHETRSGFLAKAALLTMNQA

Nucleotide

• Length: 402 bp

>AT22973 NC_011035:1211251-1211652 [Neisseria gonorrhoeae NCCP11945]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Toxin

• AlphaFold DB: Q5F6D1

Antitoxin

• AlphaFold DB: Q5F6D2