Detailed information of TA system TA229729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 888318..888852
• Replicon: chromosome
• Accession: NZ_CP089934
• Organism: Erwinia tracheiphila strain SCR3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6P1V8P2
• Locus tag: LU632_RS04530
• Protein ID: WP_000143805.1
• Coordinates: 888562..888852 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7I6XZR7
• Locus tag: LU632_RS04525
• Protein ID: WP_000104338.1
• Coordinates: 888318..888572 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU632_RS04490 (LU632_04490)	883701..884051	-	351	WP_233480010.1	hypothetical protein	-
LU632_RS04495 (LU632_04495)	884011..884283	-	273	WP_233480011.1	hypothetical protein	-
LU632_RS04500 (LU632_04500)	884522..884725	+	204	WP_233480897.1	hypothetical protein	-
LU632_RS04505 (LU632_04505)	885133..885585	-	453	WP_233480012.1	H-NS family nucleoid-associated regulatory protein	-
LU632_RS04510 (LU632_04510)	885733..886956	-	1224	WP_233478977.1	IS91 family transposase	-
LU632_RS04515 (LU632_04515)	887038..887541	-	504	Protein_888	DUF4158 domain-containing protein	-
LU632_RS04520 (LU632_04520)	887651..887941	+	291	WP_233480013.1	hypothetical protein	-
LU632_RS04525 (LU632_04525)	888318..888572	+	255	WP_000104338.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LU632_RS04530 (LU632_04530)	888562..888852	+	291	WP_000143805.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LU632_RS04535 (LU632_04535)	889196..890419	-	1224	WP_233478977.1	IS91 family transposase	-
LU632_RS04540 (LU632_04540)	890518..890937	+	420	WP_233480739.1	recombinase family protein	-
LU632_RS04545 (LU632_04545)	891176..892093	+	918	WP_233480014.1	hypothetical protein	-
LU632_RS04550 (LU632_04550)	892214..892813	+	600	WP_233480015.1	DUF2913 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hsiB1/vipA / hsiC1/vipB	746125..1186476	440351
	-	flank	IS/Tn	-	-	887020..887520	500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-47)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11463.41 Da
• Isoelectric Point: 10.2689

>T229729 WP_000143805.1 NZ_CP089934:888562-888852 [Erwinia tracheiphila]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Nucleotide

• Length: 291 bp

>T229729 NZ_CP124511:4965713-4965967 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9708.04 Da
• Isoelectric Point: 4.4247

>AT229729 WP_000104338.1 NZ_CP089934:888318-888572 [Erwinia tracheiphila]
MSTIQIRVDDELKKDAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSEDDEDADILAVVRERLKNPARRIRVN
VDDL

Nucleotide

• Length: 255 bp

>AT229729 NZ_CP124511:4965465-4965716 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A6P1V8P2

Antitoxin

• AlphaFold DB: A0A7I6XZR7