Detailed information of TA system TA229727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 312508..313139
• Replicon: chromosome
• Accession: NZ_CP089934
• Organism: Erwinia tracheiphila strain SCR3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LU632_RS01550
• Protein ID: WP_233479719.1
• Coordinates: 312508..312783 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0M2K504
• Locus tag: LU632_RS01555
• Protein ID: WP_046371849.1
• Coordinates: 312780..313139 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU632_RS01525 (LU632_01525)	308330..308536	+	207	WP_016193242.1	hypothetical protein	-
LU632_RS01530 (LU632_01530)	308868..309110	+	243	WP_016193223.1	hypothetical protein	-
LU632_RS01535 (LU632_01535)	309183..309566	+	384	WP_016193222.1	hypothetical protein	-
LU632_RS01540 (LU632_01540)	309566..312061	+	2496	WP_052734703.1	replication endonuclease	-
LU632_RS01545 (LU632_01545)	312027..312452	+	426	WP_233479718.1	hypothetical protein	-
LU632_RS01550 (LU632_01550)	312508..312783	+	276	WP_233479719.1	type II toxin-antitoxin system HicA family toxin	Toxin
LU632_RS01555 (LU632_01555)	312780..313139	+	360	WP_046371849.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LU632_RS01560 (LU632_01560)	313389..314084	+	696	WP_233479720.1	DNA adenine methylase	-
LU632_RS01565 (LU632_01565)	314151..314372	+	222	WP_006117881.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LU632_RS01570 (LU632_01570)	314369..314752	+	384	WP_046371846.1	type II toxin-antitoxin system death-on-curing family toxin	-
LU632_RS01575 (LU632_01575)	315238..315741	+	504	WP_233479721.1	hypothetical protein	-
LU632_RS01580 (LU632_01580)	315812..316870	-	1059	WP_233479722.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	301719..353787	52068

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10068.60 Da
• Isoelectric Point: 11.1780

>T229727 WP_233479719.1 NZ_CP089934:312508-312783 [Erwinia tracheiphila]
MNQAVKNLRKKQRDTLEQIFRTSPPSGLKWSAVESLIGALCGKIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGAVVSL
REWLESTGVKP

Nucleotide

• Length: 276 bp

>T229727 NZ_CP124511:3942734-3943141 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13355.08 Da
• Isoelectric Point: 4.5809

>AT229727 WP_046371849.1 NZ_CP089934:312780-313139 [Erwinia tracheiphila]
MTKPATPNMIVIAGQPAVVSYVPEINMFRGRFLGLTGYCDFVSDSIQGLNKEGDISLREYLEDCSAEGIEPYARQEKTRT
FTLRYPESFGERLNQAAAENEVSVNAFIVETLNERMKHA

Nucleotide

• Length: 360 bp

>AT229727 NZ_CP124511:3942487-3942753 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2K504