Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PumA-relB/upstrm_HI1419-dnstrm_HI1420 |
| Location | 4844683..4845265 | Replicon | chromosome |
| Accession | NZ_CP089932 | ||
| Organism | Erwinia tracheiphila strain BHKY | ||
Toxin (Protein)
| Gene name | PumA | Uniprot ID | A0A0M2KC79 |
| Locus tag | LU633_RS25050 | Protein ID | WP_016192358.1 |
| Coordinates | 4844683..4844979 (+) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | E8PSG1 |
| Locus tag | LU633_RS25055 | Protein ID | WP_013583077.1 |
| Coordinates | 4844981..4845265 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LU633_RS25020 (LU633_25020) | 4840467..4841057 | - | 591 | WP_016192352.1 | type III effector protein | - |
| LU633_RS25025 (LU633_25025) | 4841128..4841385 | - | 258 | WP_016192353.1 | hypothetical protein | - |
| LU633_RS25030 (LU633_25030) | 4841562..4842679 | + | 1118 | Protein_4926 | RNA-guided endonuclease TnpB family protein | - |
| LU633_RS25035 (LU633_25035) | 4843060..4843350 | + | 291 | WP_232426910.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LU633_RS25040 (LU633_25040) | 4843343..4843660 | + | 318 | WP_016192357.1 | helix-turn-helix domain-containing protein | - |
| LU633_RS25045 (LU633_25045) | 4843767..4844472 | + | 706 | Protein_4929 | IS6 family transposase | - |
| LU633_RS25050 (LU633_25050) | 4844683..4844979 | + | 297 | WP_016192358.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LU633_RS25055 (LU633_25055) | 4844981..4845265 | + | 285 | WP_013583077.1 | putative addiction module antidote protein | Antitoxin |
| LU633_RS25060 (LU633_25060) | 4845565..4846605 | + | 1041 | WP_046371791.1 | IS481 family transposase | - |
| LU633_RS25065 (LU633_25065) | 4846925..4848042 | + | 1118 | Protein_4933 | RNA-guided endonuclease TnpB family protein | - |
| LU633_RS25070 (LU633_25070) | 4848136..4848555 | + | 420 | WP_016192361.1 | protein rep | - |
| LU633_RS25075 (LU633_25075) | 4848625..4849233 | + | 609 | WP_016192362.1 | pesticin C-terminus-like muramidase | - |
| LU633_RS25080 (LU633_25080) | 4849230..4849643 | + | 414 | WP_016192363.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | ospG | 4819245..4897138 | 77893 | |
| - | inside | IScluster/Tn | - | ospG | 4839634..4878745 | 39111 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11248.10 Da Isoelectric Point: 10.3896
>T229725 WP_016192358.1 NZ_CP089932:4844683-4844979 [Erwinia tracheiphila]
MIEIKRTPEVEKWLKSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRVHQGKGYRVYFGNKNNKIILLLCGGDKS
TQQADIRKAKQLAKQWGF
MIEIKRTPEVEKWLKSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRVHQGKGYRVYFGNKNNKIILLLCGGDKS
TQQADIRKAKQLAKQWGF
Download Length: 297 bp
>T229725 NZ_CP124511:3612288-3612602 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10576.01 Da Isoelectric Point: 4.7254
>AT229725 WP_013583077.1 NZ_CP089932:4844981-4845265 [Erwinia tracheiphila]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNMSQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNMSQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA
Download Length: 285 bp
>AT229725 NZ_CP124511:3612599-3613015 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M2KC79 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M2KB14 |