Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 4844683..4845265 Replicon chromosome
Accession NZ_CP089932
Organism Erwinia tracheiphila strain BHKY

Toxin (Protein)


Gene name PumA Uniprot ID A0A0M2KC79
Locus tag LU633_RS25050 Protein ID WP_016192358.1
Coordinates 4844683..4844979 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID E8PSG1
Locus tag LU633_RS25055 Protein ID WP_013583077.1
Coordinates 4844981..4845265 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU633_RS25020 (LU633_25020) 4840467..4841057 - 591 WP_016192352.1 type III effector protein -
LU633_RS25025 (LU633_25025) 4841128..4841385 - 258 WP_016192353.1 hypothetical protein -
LU633_RS25030 (LU633_25030) 4841562..4842679 + 1118 Protein_4926 RNA-guided endonuclease TnpB family protein -
LU633_RS25035 (LU633_25035) 4843060..4843350 + 291 WP_232426910.1 type II toxin-antitoxin system RelE/ParE family toxin -
LU633_RS25040 (LU633_25040) 4843343..4843660 + 318 WP_016192357.1 helix-turn-helix domain-containing protein -
LU633_RS25045 (LU633_25045) 4843767..4844472 + 706 Protein_4929 IS6 family transposase -
LU633_RS25050 (LU633_25050) 4844683..4844979 + 297 WP_016192358.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LU633_RS25055 (LU633_25055) 4844981..4845265 + 285 WP_013583077.1 putative addiction module antidote protein Antitoxin
LU633_RS25060 (LU633_25060) 4845565..4846605 + 1041 WP_046371791.1 IS481 family transposase -
LU633_RS25065 (LU633_25065) 4846925..4848042 + 1118 Protein_4933 RNA-guided endonuclease TnpB family protein -
LU633_RS25070 (LU633_25070) 4848136..4848555 + 420 WP_016192361.1 protein rep -
LU633_RS25075 (LU633_25075) 4848625..4849233 + 609 WP_016192362.1 pesticin C-terminus-like muramidase -
LU633_RS25080 (LU633_25080) 4849230..4849643 + 414 WP_016192363.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - ospG 4819245..4897138 77893
- inside IScluster/Tn - ospG 4839634..4878745 39111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Uncharacterized protein HI1420

(4-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11248.10 Da        Isoelectric Point: 10.3896

>T229725 WP_016192358.1 NZ_CP089932:4844683-4844979 [Erwinia tracheiphila]
MIEIKRTPEVEKWLKSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRVHQGKGYRVYFGNKNNKIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Download         Length: 297 bp

>T229725 NZ_CP124511:3612288-3612602 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10576.01 Da        Isoelectric Point: 4.7254

>AT229725 WP_013583077.1 NZ_CP089932:4844981-4845265 [Erwinia tracheiphila]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNMSQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Download         Length: 285 bp

>AT229725 NZ_CP124511:3612599-3613015 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2KC79

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2KB14

References