Detailed information of TA system TA229724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 4843060..4843660
• Replicon: chromosome
• Accession: NZ_CP089932
• Organism: Erwinia tracheiphila strain BHKY

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LU633_RS25035
• Protein ID: WP_232426910.1
• Coordinates: 4843060..4843350 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LU633_RS25040
• Protein ID: WP_016192357.1
• Coordinates: 4843343..4843660 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU633_RS25015 (LU633_25015)	4839634..4840338	-	705	WP_046371935.1	IS6 family transposase	-
LU633_RS25020 (LU633_25020)	4840467..4841057	-	591	WP_016192352.1	type III effector protein	-
LU633_RS25025 (LU633_25025)	4841128..4841385	-	258	WP_016192353.1	hypothetical protein	-
LU633_RS25030 (LU633_25030)	4841562..4842679	+	1118	Protein_4926	RNA-guided endonuclease TnpB family protein	-
LU633_RS25035 (LU633_25035)	4843060..4843350	+	291	WP_232426910.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LU633_RS25040 (LU633_25040)	4843343..4843660	+	318	WP_016192357.1	helix-turn-helix domain-containing protein	Antitoxin
LU633_RS25045 (LU633_25045)	4843767..4844472	+	706	Protein_4929	IS6 family transposase	-
LU633_RS25050 (LU633_25050)	4844683..4844979	+	297	WP_016192358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LU633_RS25055 (LU633_25055)	4844981..4845265	+	285	WP_013583077.1	putative addiction module antidote protein	-
LU633_RS25060 (LU633_25060)	4845565..4846605	+	1041	WP_046371791.1	IS481 family transposase	-
LU633_RS25065 (LU633_25065)	4846925..4848042	+	1118	Protein_4933	RNA-guided endonuclease TnpB family protein	-
LU633_RS25070 (LU633_25070)	4848136..4848555	+	420	WP_016192361.1	protein rep	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ospG	4819245..4897138	77893
	-	inside	IScluster/Tn	-	ospG	4839634..4878745	39111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (6-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10687.23 Da
• Isoelectric Point: 4.6484

>T229724 WP_232426910.1 NZ_CP089932:4843060-4843350 [Erwinia tracheiphila]
MPGDPLGKFTFKKRLGLPGVGARDGARSIVFFNDGDNIFFFDMYLKSGLSKKKGKELEDDEIDAYCNIAKDFISMSPATI
NKLLSDKELIEVNCDD

Nucleotide

• Length: 291 bp

>T229724 NZ_CP124511:c3572225-3571761 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11590.53 Da
• Isoelectric Point: 10.8279

>AT229724 WP_016192357.1 NZ_CP089932:4843343-4843660 [Erwinia tracheiphila]
MTERLKRIQGMAERYNKLGVVKDETVAAIDAAVAARNIPAIRVMTGSQIKEVRTRYNMSQAALAITTGMSVESVSKWERN
ESKPNKAALRIINIIDAKGPQVFMA

Nucleotide

• Length: 318 bp

>AT229724 NZ_CP124511:c3572560-3572225 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA