Detailed information of TA system TA229712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1858168..1858745
• Replicon: chromosome
• Accession: NZ_CP089932
• Organism: Erwinia tracheiphila strain BHKY

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0M2KF28
• Locus tag: LU633_RS09780
• Protein ID: WP_016192194.1
• Coordinates: 1858434..1858745 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0M2K853
• Locus tag: LU633_RS09775
• Protein ID: WP_016192195.1
• Coordinates: 1858168..1858446 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU633_RS09740 (LU633_09740)	1854077..1854529	-	453	Protein_1903	IS6 family transposase	-
LU633_RS09745 (LU633_09745)	1854524..1855267	-	744	Protein_1904	transposase	-
LU633_RS09750 (LU633_09750)	1855361..1855834	-	474	WP_016192197.1	DUF3757 domain-containing protein	-
LU633_RS09755 (LU633_09755)	1855989..1856213	-	225	WP_051124401.1	hypothetical protein	-
LU633_RS09760 (LU633_09760)	1856270..1856422	-	153	WP_161796973.1	hypothetical protein	-
LU633_RS09765 (LU633_09765)	1856563..1856736	+	174	Protein_1908	hypothetical protein	-
LU633_RS09770 (LU633_09770)	1856800..1857906	+	1107	WP_233482028.1	IS91 family transposase	-
LU633_RS09775 (LU633_09775)	1858168..1858446	+	279	WP_016192195.1	CopG family ribbon-helix-helix protein	Antitoxin
LU633_RS09780 (LU633_09780)	1858434..1858745	+	312	WP_016192194.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LU633_RS09785 (LU633_09785)	1858871..1859305	+	435	Protein_1912	DDE-type integrase/transposase/recombinase	-
LU633_RS09790 (LU633_09790)	1859470..1860687	-	1218	WP_040465737.1	IS91 family transposase	-
LU633_RS09795 (LU633_09795)	1860674..1860904	-	231	WP_046371992.1	hypothetical protein	-
LU633_RS09800 (LU633_09800)	1861023..1861259	+	237	Protein_1915	transposase	-
LU633_RS09805 (LU633_09805)	1861333..1862030	+	698	WP_152664192.1	IS1 family transposase	-
LU633_RS09810 (LU633_09810)	1862134..1862580	-	447	WP_071598937.1	FAD-dependent oxidoreductase	-
LU633_RS09815 (LU633_09815)	1862577..1863290	-	714	WP_016192189.1	FAD-dependent oxidoreductase	-
LU633_RS09820 (LU633_09820)	1863283..1863594	-	312	WP_016192188.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1855989..1880440	24451
	-	inside	IScluster/Tn	-	-	1856563..1862030	5467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11746.62 Da
• Isoelectric Point: 9.4808

>T229712 WP_016192194.1 NZ_CP089932:1858434-1858745 [Erwinia tracheiphila]
MPQVIVSALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKTQPDMGRPVSLLPLEYQELVIGFGDSGYVMLYRHDR
ETDQIVILTALHQKEAGYRGLQE

Nucleotide

• Length: 312 bp

>T229712 NZ_CP124511:678067-678174 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10727.70 Da
• Isoelectric Point: 4.6216

>AT229712 WP_016192195.1 NZ_CP089932:1858168-1858446 [Erwinia tracheiphila]
MSAATSIRLDDELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLSAEEVESWIDTWG
TENEQDAPSCHR

Nucleotide

• Length: 279 bp

>AT229712 NZ_CP124511:c678010-677955 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Structures

Toxin

• AlphaFold DB: A0A0M2KF28

Antitoxin

• AlphaFold DB: A0A0M2K853