Detailed information of TA system TA229707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4346247..4346866
• Replicon: chromosome
• Accession: NZ_CP089927
• Organism: Mycobacterium tuberculosis strain M20

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LV451_RS20510
• Protein ID: WP_003404726.1
• Coordinates: 4346432..4346866 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: LV451_RS20505
• Protein ID: WP_003898641.1
• Coordinates: 4346247..4346426 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV451_RS20490	4341720..4343300	+	1581	WP_003909313.1	serine hydrolase	-
LV451_RS20495	4343297..4345690	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LV451_RS20500	4345963..4346121	+	159	WP_003404720.1	hypothetical protein	-
LV451_RS20505	4346247..4346426	+	180	WP_003898641.1	antitoxin	Antitoxin
LV451_RS20510	4346432..4346866	+	435	WP_003404726.1	SRPBCC family protein	Toxin
LV451_RS20515	4346964..4347737	+	774	WP_003404735.1	VOC family protein	-
LV451_RS20520	4347802..4348251	+	450	WP_003404738.1	hypothetical protein	-
LV451_RS20525	4348386..4348616	-	231	WP_003898642.1	hypothetical protein	-
LV451_RS20530	4348783..4350291	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LV451_RS20535	4350293..4351531	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T229707 WP_003404726.1 NZ_CP089927:4346432-4346866 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T229707 NZ_CP124508:c4464518-4464345 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT229707 WP_003898641.1 NZ_CP089927:4346247-4346426 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT229707 NZ_CP124508:c4464842-4464546 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5