Detailed information of TA system TA229706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4173934..4174643
• Replicon: chromosome
• Accession: NZ_CP089927
• Organism: Mycobacterium tuberculosis strain M20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF74
• Locus tag: LV451_RS19665
• Protein ID: WP_003403837.1
• Coordinates: 4174215..4174643 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TEX4
• Locus tag: LV451_RS19660
• Protein ID: WP_003403834.1
• Coordinates: 4173934..4174191 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV451_RS19635	4169223..4169729	-	507	WP_003403827.1	helix-turn-helix domain-containing protein	-
LV451_RS19640	4169842..4169985	-	144	Protein_3882	3-hydroxyisobutyryl-CoA hydrolase	-
LV451_RS19645	4169997..4170241	+	245	Protein_3883	DUF1998 domain-containing protein	-
LV451_RS20855	4170484..4171226	+	743	Protein_3884	PE family protein	-
LV451_RS20860	4171324..4171722	+	399	WP_277884384.1	PE-PGRS family protein	-
LV451_RS19655	4172121..4173843	+	1723	Protein_3886	PE family protein	-
LV451_RS19660	4173934..4174191	+	258	WP_003403834.1	ribbon-helix-helix protein, CopG family	Antitoxin
LV451_RS19665	4174215..4174643	+	429	WP_003403837.1	type II toxin-antitoxin system VapC family toxin	Toxin
LV451_RS19670	4174724..4174861	-	138	WP_003403839.1	hypothetical protein	-
LV451_RS19680	4175020..4175265	+	246	WP_003403841.1	hypothetical protein	-
LV451_RS19685	4175334..4176218	-	885	WP_003403844.1	3-hydroxyisobutyrate dehydrogenase	-
LV451_RS19690	4176229..4177401	-	1173	WP_003403845.1	acyl-CoA dehydrogenase family protein	-
LV451_RS19695	4177408..4178940	-	1533	WP_003403847.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15831.27 Da
• Isoelectric Point: 7.5536

>T229706 WP_003403837.1 NZ_CP089927:4174215-4174643 [Mycobacterium tuberculosis]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Nucleotide

• Length: 429 bp

>T229706 NZ_CP124508:4452744-4453121 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9114.28 Da
• Isoelectric Point: 10.6530

>AT229706 WP_003403834.1 NZ_CP089927:4173934-4174191 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT229706 NZ_CP124508:4452286-4452654 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829CDR3

Antitoxin

• AlphaFold DB: G0TEX4