Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 4089773..4090565 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag LV451_RS19185 Protein ID WP_003403386.1
Coordinates 4090128..4090565 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LV451_RS19180 Protein ID WP_003403381.1
Coordinates 4089773..4090018 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS19150 4084793..4086298 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LV451_RS19155 4086379..4087389 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LV451_RS19160 4087777..4088160 - 384 WP_003403365.1 ribonuclease VapC6 -
LV451_RS19165 4088255..4088410 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LV451_RS19170 4088486..4089202 - 717 WP_010886082.1 CPBP family intramembrane metalloprotease -
LV451_RS19175 4089478..4089786 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
LV451_RS19180 4089773..4090018 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LV451_RS19185 4090128..4090565 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
LV451_RS19190 4090562..4090816 - 255 WP_003911263.1 antitoxin VapB7 -
LV451_RS19195 4090930..4093293 + 2364 WP_003898547.1 arylsulfatase AtsD -
LV451_RS19200 4093356..4093682 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LV451_RS19205 4093594..4093932 + 339 WP_003403405.1 PIN domain-containing protein -
LV451_RS19210 4093929..4094102 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T229704 WP_003403386.1 NZ_CP089927:c4090565-4090128 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T229704 NZ_CP124508:c4069430-4069143 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT229704 WP_003403381.1 NZ_CP089927:c4090018-4089773 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT229704 NZ_CP124508:c4069156-4068854 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References