Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 4029640..4030286 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag LV451_RS18820 Protein ID WP_003403122.1
Coordinates 4029640..4030032 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag LV451_RS18825 Protein ID WP_003403125.1
Coordinates 4030029..4030286 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS18805 4025302..4026828 + 1527 WP_003403112.1 virulence factor Mce family protein -
LV451_RS18810 4026891..4028033 + 1143 WP_003911253.1 virulence factor Mce family protein -
LV451_RS18815 4028038..4029588 + 1551 WP_003403119.1 MlaD family protein -
LV451_RS18820 4029640..4030032 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
LV451_RS18825 4030029..4030286 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
LV451_RS18830 4030469..4031704 - 1236 WP_003403128.1 ATP-binding protein -
LV451_RS18835 4031955..4032368 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
LV451_RS18840 4032365..4032601 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
LV451_RS18845 4032705..4033211 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LV451_RS18850 4033325..4033855 - 531 WP_003917320.1 two component system sensor kinase HK2 -
LV451_RS18855 4033839..4034534 - 696 WP_003918099.1 two-component system response regulator TcrA -
LV451_RS18860 4034657..4034968 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T229696 WP_003403122.1 NZ_CP089927:c4030032-4029640 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T229696 NZ_CP124508:2180060-2180162 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT229696 WP_003403125.1 NZ_CP089927:c4030286-4030029 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT229696 NZ_CP124508:c2180200-2179932 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References