Detailed information of TA system TA229694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 3975366..3976042
• Replicon: chromosome
• Accession: NZ_CP089927
• Organism: Mycobacterium tuberculosis strain M20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: LV451_RS18585
• Protein ID: WP_003898500.1
• Coordinates: 3975366..3975779 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: LV451_RS18590
• Protein ID: WP_003402915.1
• Coordinates: 3975776..3976042 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV451_RS18555	3970711..3971013	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
LV451_RS18560	3971073..3971351	-	279	WP_003402901.1	hypothetical protein	-
LV451_RS18565	3971348..3972601	-	1254	WP_003911224.1	inorganic phosphate transporter	-
LV451_RS18570	3972721..3973107	-	387	WP_003402907.1	VOC family protein	-
LV451_RS18575	3973170..3974054	-	885	WP_003402909.1	SDR family oxidoreductase	-
LV451_RS18580	3974150..3975052	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
LV451_RS18585	3975366..3975779	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
LV451_RS18590	3975776..3976042	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LV451_RS18595	3976234..3977949	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
LV451_RS18600	3978027..3979631	+	1605	WP_003402920.1	amidohydrolase	-
LV451_RS18605	3979628..3980608	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T229694 WP_003898500.1 NZ_CP089927:c3975779-3975366 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T229694 NZ_CP124508:1923948-1924202 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT229694 WP_003402915.1 NZ_CP089927:c3976042-3975776 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT229694 NZ_CP124508:1923700-1923951 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0