Detailed information of TA system TA229690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 3699100..3699626
• Replicon: chromosome
• Accession: NZ_CP089927
• Organism: Mycobacterium tuberculosis strain M20

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LV451_RS17275
• Protein ID: WP_003401560.1
• Coordinates: 3699324..3699626 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: LV451_RS17270
• Protein ID: WP_003401555.1
• Coordinates: 3699100..3699327 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV451_RS17255	3694820..3695623	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LV451_RS17260	3695633..3697030	-	1398	WP_003401544.1	sulfatase	-
LV451_RS17265	3697209..3698957	+	1749	WP_235270669.1	PE family protein	-
LV451_RS17270	3699100..3699327	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
LV451_RS17275	3699324..3699626	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LV451_RS17280	3699674..3699895	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
LV451_RS17285	3699892..3700317	+	426	WP_003401566.1	PIN domain nuclease	-
LV451_RS17290	3700453..3701085	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LV451_RS17295	3701082..3701990	+	909	WP_003900117.1	protochlorophyllide reductase	-
LV451_RS17300	3701998..3702588	-	591	WP_229298012.1	hypothetical protein	-
LV451_RS17305	3702581..3702631	-	51	Protein_3419	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T229690 WP_003401560.1 NZ_CP089927:3699324-3699626 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T229690 NZ_CP124508:645572-645886 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT229690 WP_003401555.1 NZ_CP089927:3699100-3699327 [Mycobacterium tuberculosis]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT229690 NZ_CP124508:645883-646299 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1