Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 2061849..2062453 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag LV451_RS09950 Protein ID WP_003414492.1
Coordinates 2061849..2062241 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P71622
Locus tag LV451_RS09955 Protein ID WP_003912003.1
Coordinates 2062238..2062453 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS09920 2056999..2057787 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
LV451_RS09925 2058121..2058666 - 546 WP_003913758.1 DUF1802 family protein -
LV451_RS09930 2058938..2059822 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LV451_RS09935 2059825..2060712 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
LV451_RS09940 2061017..2061562 - 546 WP_003899500.1 DUF1802 family protein -
LV451_RS09945 2061559..2061828 - 270 WP_003414489.1 DUF2277 family protein -
LV451_RS09950 2061849..2062241 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
LV451_RS09955 2062238..2062453 - 216 WP_003912003.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LV451_RS09960 2062500..2063249 + 750 WP_003414497.1 enoyl-CoA hydratase -
LV451_RS09965 2063328..2064410 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
LV451_RS09970 2064403..2065713 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
LV451_RS09975 2065716..2066543 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
LV451_RS09980 2066540..2067451 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T229681 WP_003414492.1 NZ_CP089927:c2062241-2061849 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T229681 NZ_CP124506:c4110754-4110467 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7483.50 Da        Isoelectric Point: 4.9732

>AT229681 WP_003912003.1 NZ_CP089927:c2062453-2062238 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Download         Length: 216 bp

>AT229681 NZ_CP124506:c4110480-4110178 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB P71622

References