Detailed information of TA system TA229678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1995886..1996573
• Replicon: chromosome
• Accession: NZ_CP089927
• Organism: Mycobacterium tuberculosis strain M20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: LV451_RS09585
• Protein ID: WP_003414064.1
• Coordinates: 1996178..1996573 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: LV451_RS09580
• Protein ID: WP_003414061.1
• Coordinates: 1995886..1996152 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV451_RS09550	1991525..1992427	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LV451_RS09555	1992496..1993248	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
LV451_RS09565	1993492..1993767	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
LV451_RS09570	1993764..1995386	-	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
LV451_RS09575	1995473..1995889	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
LV451_RS09580	1995886..1996152	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LV451_RS09585	1996178..1996573	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
LV451_RS09590	1996570..1996839	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
LV451_RS09595	1996849..1997943	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
LV451_RS09600	1997940..1998359	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
LV451_RS09605	1998358..1998432	+	75	Protein_1894	hypothetical protein	-
LV451_RS09610	1998433..1998912	-	480	WP_003414073.1	dihydrofolate reductase	-
LV451_RS09615	1998983..1999783	-	801	WP_003911953.1	thymidylate synthase	-
LV451_RS09620	1999939..2000676	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T229678 WP_003414064.1 NZ_CP089927:c1996573-1996178 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T229678 NZ_CP124506:3588281-3588499 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT229678 WP_003414061.1 NZ_CP089927:c1996152-1995886 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT229678 NZ_CP124506:3587881-3588255 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64