Detailed information of TA system TA229671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1780099..1780739
• Replicon: chromosome
• Accession: NZ_CP089927
• Organism: Mycobacterium tuberculosis strain M20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LV451_RS08375
• Protein ID: WP_003412970.1
• Coordinates: 1780099..1780518 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LV451_RS08380
• Protein ID: WP_003412975.1
• Coordinates: 1780515..1780739 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV451_RS08345	1775684..1776406	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LV451_RS08350	1776923..1777150	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
LV451_RS08355	1777147..1777548	+	402	WP_003412963.1	PIN domain-containing protein	-
LV451_RS08360	1777583..1778503	-	921	WP_003412965.1	restriction endonuclease	-
LV451_RS08365	1778844..1779089	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LV451_RS08370	1779148..1780098	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LV451_RS08375	1780099..1780518	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LV451_RS08380	1780515..1780739	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LV451_RS08385	1780770..1783613	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
LV451_RS08390	1783685..1784086	-	402	WP_003412981.1	hypothetical protein	-
LV451_RS08395	1784086..1784556	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LV451_RS08400	1784559..1785122	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T229671 WP_003412970.1 NZ_CP089927:c1780518-1780099 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T229671 NZ_CP124506:1972621-1972875 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT229671 WP_003412975.1 NZ_CP089927:c1780739-1780515 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT229671 NZ_CP124506:1972373-1972624 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09