Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1155978..1156564 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag LV451_RS05425 Protein ID WP_003410010.1
Coordinates 1155978..1156322 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag LV451_RS05430 Protein ID WP_003410014.1
Coordinates 1156316..1156564 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS05390 1151684..1152283 + 600 WP_003409989.1 L-lysine exporter -
LV451_RS05395 1152699..1153127 + 429 WP_003409992.1 cellulose-binding protein -
LV451_RS05400 1153353..1153892 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LV451_RS05405 1154412..1154972 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
LV451_RS05410 1154969..1155310 - 342 WP_003410003.1 DUF2384 domain-containing protein -
LV451_RS05415 1155396..1155653 + 258 WP_003410006.1 hypothetical protein -
LV451_RS05420 1155554..1155889 - 336 WP_003410009.1 dehydrogenase -
LV451_RS05425 1155978..1156322 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
LV451_RS05430 1156316..1156564 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
LV451_RS05435 1156664..1158979 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LV451_RS05440 1158976..1159248 - 273 WP_003410017.1 DUF1490 family protein -
LV451_RS05445 1159301..1159657 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
LV451_RS05450 1159814..1160581 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T229664 WP_003410010.1 NZ_CP089927:c1156322-1155978 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T229664 NZ_CP124506:188541-188648 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGTTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT229664 WP_003410014.1 NZ_CP089927:c1156564-1156316 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT229664 NZ_CP124506:c188484-188427 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References