Detailed information of TA system TA022966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 4676145..4676859
• Replicon: chromosome
• Accession: NC_011004
• Organism: Rhodopseudomonas palustris TIE-1

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: RPAL_RS21530
• Protein ID: WP_012497200.1
• Coordinates: 4676476..4676859 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B3QI46
• Locus tag: RPAL_RS21525
• Protein ID: WP_012497199.1
• Coordinates: 4676145..4676495 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPAL_RS21495	4671707..4672408	-	702	WP_012497195.1	phosphoribosylformylglycinamidine synthase subunit PurQ	-
RPAL_RS21500	4672465..4672704	-	240	WP_011159357.1	hypothetical protein	-
RPAL_RS21505	4672728..4672973	-	246	WP_011159358.1	phosphoribosylformylglycinamidine synthase subunit PurS	-
RPAL_RS21510	4673156..4673923	-	768	WP_011159359.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RPAL_RS21515	4674222..4674545	+	324	WP_012497197.1	DUF1476 domain-containing protein	-
RPAL_RS21520	4675117..4675791	+	675	WP_012497198.1	hypothetical protein	-
RPAL_RS21525	4676145..4676495	-	351	WP_012497199.1	helix-turn-helix domain-containing protein	Antitoxin
RPAL_RS21530	4676476..4676859	-	384	WP_012497200.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RPAL_RS21535	4676968..4677786	-	819	WP_012497201.1	isocitrate lyase/phosphoenolpyruvate mutase family protein	-
RPAL_RS21540	4678103..4678486	-	384	WP_012497202.1	hypothetical protein	-
RPAL_RS21545	4678782..4679021	+	240	WP_012497203.1	hypothetical protein	-
RPAL_RS21555	4680442..4680996	-	555	WP_012497205.1	hypothetical protein	-
RPAL_RS21560	4681067..4681549	-	483	WP_012497206.1	transcription elongation factor GreA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (20-112)

Antitoxin

• HigA2-like, helix-turn-helix domain (21-100)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14384.36 Da
• Isoelectric Point: 10.1303

>T22966 WP_012497200.1 NC_011004:c4676859-4676476 [Rhodopseudomonas palustris TIE-1]
MRPRLVSGEKPLHWVGSSKDDLCAFPLPVQREIGNALGLAQFGGKHPRAKPWKGEGPGVFEMVDDFDGDTYRAVYTVRFR
HAVYVLHAFQKKSPRGIRTARGDIELIARRLKLARQDDEARDGENDR

Nucleotide

• Length: 384 bp

>T22966 NC_011004:c4676859-4676476 [Rhodopseudomonas palustris TIE-1]
ATGCGACCGCGACTTGTAAGCGGCGAAAAGCCGCTCCACTGGGTGGGCTCATCGAAAGACGACCTTTGCGCCTTTCCGTT
GCCGGTGCAGCGTGAGATCGGCAATGCGCTGGGATTGGCGCAGTTCGGCGGCAAGCATCCGCGTGCCAAGCCCTGGAAGG
GCGAGGGGCCGGGTGTGTTCGAAATGGTCGACGATTTCGACGGCGATACCTACCGCGCGGTCTATACGGTGCGCTTCCGG
CACGCGGTCTACGTGCTGCACGCATTCCAGAAGAAGTCGCCGCGCGGCATCCGAACCGCGCGCGGCGATATCGAACTCAT
TGCGCGGCGGCTGAAGCTCGCCCGACAGGACGACGAGGCTCGTGATGGCGAGAACGACCGCTAG

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 12564.57 Da
• Isoelectric Point: 11.2526

>AT22966 WP_012497199.1 NC_011004:c4676495-4676145 [Rhodopseudomonas palustris TIE-1]
MARTTASKGTAAEITRGSGNVFADLGMPDAEERQTKLRLAYALNVVIDRARLSQAAAAARLGINQPKVSALRNYKLEGFS
VERLMTLLNALDQDIEIIIRKKPRSRAAARISVVAA

Nucleotide

• Length: 351 bp

>AT22966 NC_011004:c4676495-4676145 [Rhodopseudomonas palustris TIE-1]
ATGGCGAGAACGACCGCTAGCAAAGGCACGGCAGCCGAGATCACCCGCGGCAGCGGCAATGTGTTTGCGGACCTGGGCAT
GCCCGATGCGGAGGAGAGGCAGACCAAGCTCAGGCTCGCCTACGCTTTGAACGTGGTGATCGACCGCGCTCGCCTGAGCC
AAGCCGCCGCTGCCGCGCGGCTCGGGATCAACCAGCCCAAGGTTTCGGCCCTGCGCAACTACAAACTGGAAGGCTTTTCG
GTCGAGCGGCTGATGACGTTGCTCAACGCGCTCGATCAGGACATCGAAATTATCATCCGCAAAAAGCCACGCTCGCGCGC
GGCGGCGCGGATCAGCGTAGTGGCGGCGTGA

Structures

Antitoxin

• AlphaFold DB: B3QI46