Detailed information of TA system TA229659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1116316..1117019
• Replicon: chromosome
• Accession: NZ_CP089927
• Organism: Mycobacterium tuberculosis strain M20

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: LV451_RS05150
• Protein ID: WP_003409778.1
• Coordinates: 1116316..1116645 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: LV451_RS05155
• Protein ID: WP_003409780.1
• Coordinates: 1116642..1117019 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV451_RS05130	1112699..1113769	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
LV451_RS05135	1113766..1114281	+	516	WP_003409718.1	flavin reductase family protein	-
LV451_RS05140	1114278..1115339	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LV451_RS05145	1115336..1116106	+	771	WP_003409775.1	SDR family oxidoreductase	-
LV451_RS05150	1116316..1116645	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LV451_RS05155	1116642..1117019	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
LV451_RS05160	1117016..1117606	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
LV451_RS05165	1117661..1119025	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
LV451_RS05170	1119180..1119632	-	453	WP_003899095.1	lipoprotein	-
LV451_RS05175	1119696..1120097	+	402	WP_003409869.1	hypothetical protein	-
LV451_RS05180	1120090..1120272	-	183	WP_003409870.1	hypothetical protein	-
LV451_RS05185	1120386..1120736	-	351	WP_003899096.1	hypothetical protein	-
LV451_RS05190	1120747..1121649	-	903	WP_003899097.1	hypothetical protein	-
LV451_RS05195	1121670..1121861	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T229659 WP_003409778.1 NZ_CP089927:c1116645-1116316 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T229659 NZ_CP124502:4473129-4473593 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT229659 WP_003409780.1 NZ_CP089927:c1117019-1116642 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT229659 NZ_CP124502:4472794-4473129 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8