Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 871094..871707 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag LV451_RS04030 Protein ID WP_003408465.1
Coordinates 871094..871483 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag LV451_RS04035 Protein ID WP_003408469.1
Coordinates 871480..871707 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS03995 866723..867637 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
LV451_RS04000 867640..868470 + 831 WP_003916848.1 cyclase family protein -
LV451_RS04005 868470..868820 + 351 WP_003898986.1 cupin domain-containing protein -
LV451_RS04010 868873..869691 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
LV451_RS04015 869705..870484 + 780 WP_003408460.1 IclR family transcriptional regulator -
LV451_RS04025 870915..871046 + 132 Protein_792 IS3 family transposase -
LV451_RS04030 871094..871483 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
LV451_RS04035 871480..871707 - 228 WP_003408469.1 antitoxin Antitoxin
LV451_RS04040 871925..873409 + 1485 WP_003408473.1 biotin carboxylase -
LV451_RS04045 873406..874653 + 1248 WP_003408476.1 serine hydrolase -
LV451_RS04050 874696..875115 - 420 WP_003408483.1 hypothetical protein -
LV451_RS04055 875105..875815 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T229657 WP_003408465.1 NZ_CP089927:c871483-871094 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T229657 NZ_CP124502:4253058-4253435 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT229657 WP_003408469.1 NZ_CP089927:c871707-871480 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT229657 NZ_CP124502:4252601-4252981 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References