Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 499030..499685 Replicon chromosome
Accession NZ_CP089927
Organism Mycobacterium tuberculosis strain M20

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA6
Locus tag LV451_RS02360 Protein ID WP_003898861.1
Coordinates 499030..499431 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag LV451_RS02365 Protein ID WP_003407272.1
Coordinates 499428..499685 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV451_RS02345 494739..496124 - 1386 WP_003911514.1 cytochrome P450 -
LV451_RS02350 496202..497236 + 1035 WP_003407264.1 AraC family transcriptional regulator -
LV451_RS02355 497282..498775 - 1494 Protein_461 PE family protein -
LV451_RS02360 499030..499431 - 402 WP_003898861.1 type II toxin-antitoxin system VapC family toxin Toxin
LV451_RS02365 499428..499685 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
LV451_RS02370 499768..500727 - 960 WP_003407276.1 alpha/beta hydrolase -
LV451_RS02375 500752..501714 - 963 WP_003407279.1 alpha/beta hydrolase -
LV451_RS02380 501713..502450 + 738 WP_031647000.1 lysoplasmalogenase -
LV451_RS02385 502531..504498 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14952.43 Da        Isoelectric Point: 11.4656

>T229654 WP_003898861.1 NZ_CP089927:c499431-499030 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T229654 NZ_CP124502:c3079430-3079176 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT229654 WP_003407272.1 NZ_CP089927:c499685-499428 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT229654 NZ_CP124502:c3079678-3079427 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045ISB0

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References