Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5217007..5217632 Replicon chromosome
Accession NZ_CP089926
Organism Klebsiella pneumoniae strain KP111

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LVQ71_RS25520 Protein ID WP_002882817.1
Coordinates 5217007..5217390 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVQ71_RS25525 Protein ID WP_004150355.1
Coordinates 5217390..5217632 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ71_RS25505 (5214373) 5214373..5215275 + 903 WP_135652828.1 formate dehydrogenase subunit beta -
LVQ71_RS25510 (5215272) 5215272..5215907 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVQ71_RS25515 (5215904) 5215904..5216833 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LVQ71_RS25520 (5217007) 5217007..5217390 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LVQ71_RS25525 (5217390) 5217390..5217632 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVQ71_RS25530 (5217837) 5217837..5218754 + 918 WP_004150353.1 alpha/beta hydrolase -
LVQ71_RS25535 (5218768) 5218768..5219709 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVQ71_RS25540 (5219754) 5219754..5220191 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LVQ71_RS25545 (5220188) 5220188..5221048 - 861 WP_004146232.1 virulence factor BrkB family protein -
LVQ71_RS25550 (5221042) 5221042..5221641 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T229647 WP_002882817.1 NZ_CP089926:c5217390-5217007 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T229647 NZ_CP124502:c1456660-1456442 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT229647 WP_004150355.1 NZ_CP089926:c5217632-5217390 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT229647 NZ_CP124502:c1457060-1456686 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References