Detailed information of TA system TA229644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4569397..4570207
• Replicon: chromosome
• Accession: NZ_CP089926
• Organism: Klebsiella pneumoniae strain KP111

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: LVQ71_RS22540
• Protein ID: WP_071081553.1
• Coordinates: 4569397..4569930 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: LVQ71_RS22545
• Protein ID: WP_002887278.1
• Coordinates: 4569941..4570207 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ71_RS22535 (4568228)	4568228..4569349	+	1122	WP_016946712.1	cupin domain-containing protein	-
LVQ71_RS22540 (4569397)	4569397..4569930	-	534	WP_071081553.1	type II toxin-antitoxin system toxin KacT	Toxin
LVQ71_RS22545 (4569941)	4569941..4570207	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
LVQ71_RS22550 (4570310)	4570310..4571743	-	1434	WP_032413318.1	Cu(+)/Ag(+) sensor histidine kinase	-
LVQ71_RS22555 (4571733)	4571733..4572416	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
LVQ71_RS22560 (4572589)	4572589..4573974	+	1386	WP_032413315.1	efflux transporter outer membrane subunit	-
LVQ71_RS22565 (4573992)	4573992..4574336	+	345	WP_002887266.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19798.59 Da
• Isoelectric Point: 5.2614

>T229644 WP_071081553.1 NZ_CP089926:c4569930-4569397 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLATDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T229644 NZ_CP124502:933953-934240 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT229644 WP_002887278.1 NZ_CP089926:c4570207-4569941 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT229644 NZ_CP124502:934227-934529 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1