Detailed information of TA system TA022964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 4267075..4267730
• Replicon: chromosome
• Accession: NC_011004
• Organism: Rhodopseudomonas palustris TIE-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B3QEW5
• Locus tag: RPAL_RS19660
• Protein ID: WP_012496899.1
• Coordinates: 4267075..4267479 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3QEW6
• Locus tag: RPAL_RS19665
• Protein ID: WP_012496900.1
• Coordinates: 4267476..4267730 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPAL_RS19645	4262090..4262332	-	243	WP_012496896.1	hypothetical protein	-
RPAL_RS19650	4262669..4263892	+	1224	WP_012496897.1	efflux RND transporter periplasmic adaptor subunit	-
RPAL_RS19655	4263895..4267032	+	3138	WP_012496898.1	multidrug efflux RND transporter permease subunit	-
RPAL_RS19660	4267075..4267479	-	405	WP_012496899.1	PIN domain-containing protein	Toxin
RPAL_RS19665	4267476..4267730	-	255	WP_012496900.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RPAL_RS19675	4268318..4269514	+	1197	WP_012496901.1	phage portal protein	-
RPAL_RS19680	4269511..4270215	+	705	WP_012496902.1	tail assembly protein	-
RPAL_RS19685	4270215..4270541	+	327	WP_012496903.1	gene transfer agent family protein	-
RPAL_RS27040	4270538..4270735	+	198	WP_012496904.1	DUF2158 domain-containing protein	-
RPAL_RS19695	4270735..4271232	+	498	WP_012496905.1	HK97 family phage prohead protease	-
RPAL_RS19700	4271235..4272383	+	1149	WP_012496906.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-111)

Antitoxin

• SpoVT-AbrB domain (5-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14720.63 Da
• Isoelectric Point: 6.2105

>T22964 WP_012496899.1 NC_011004:c4267479-4267075 [Rhodopseudomonas palustris TIE-1]
VKAFFDTNILVYTTTSDPRQQTAAACLSSGGVASVQVLNEFVHVARRKLRHDWPQIEVALEQFHTALDDVLPITLTTHAA
AVVLARDHRVSFYDALIVAAAQEAGCDVLYSEALQHGRTFGSLRVENPFLEGSR

Nucleotide

• Length: 405 bp

>T22964 NC_011004:c4267479-4267075 [Rhodopseudomonas palustris TIE-1]
GTGAAGGCGTTCTTCGACACGAACATTCTCGTCTACACCACGACCTCGGACCCGCGTCAGCAAACGGCCGCTGCTTGCCT
GAGCAGCGGTGGAGTTGCCAGCGTTCAGGTGCTCAACGAATTCGTCCACGTAGCGCGCCGAAAGCTGCGGCACGACTGGC
CGCAGATCGAAGTCGCGCTCGAGCAGTTTCACACCGCGCTCGATGACGTACTTCCGATCACTCTAACCACGCATGCCGCA
GCCGTTGTCCTTGCACGCGATCACCGGGTGTCGTTCTACGATGCGCTGATCGTCGCGGCGGCCCAGGAGGCTGGCTGCGA
TGTGTTGTACAGCGAAGCCCTGCAGCACGGCCGTACCTTCGGTTCGCTGCGAGTCGAGAATCCATTTCTGGAAGGCTCGC
GATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9871.17 Da
• Isoelectric Point: 4.9432

>AT22964 WP_012496900.1 NC_011004:c4267730-4267476 [Rhodopseudomonas palustris TIE-1]
MLVSKWGNSLAVRLPKALVDKLDLKEGDELNVVDIVERTLVVEKEDRRRAAVERLSKLRWTLPADYKFDRDEANERYRDE
ANDQ

Nucleotide

• Length: 255 bp

>AT22964 NC_011004:c4267730-4267476 [Rhodopseudomonas palustris TIE-1]
ATGCTGGTTTCGAAATGGGGCAACAGTCTCGCCGTCAGGCTGCCCAAGGCGTTGGTCGACAAGCTCGATCTGAAAGAGGG
CGACGAGTTGAACGTCGTCGACATCGTCGAACGAACGCTGGTGGTTGAGAAGGAAGACCGGCGCAGGGCGGCGGTGGAGC
GCCTTTCCAAGCTGCGATGGACCCTTCCTGCTGACTACAAGTTCGATCGCGACGAGGCGAACGAGCGGTATCGCGACGAG
GCGAATGATCAGTGA

Structures

Toxin

• AlphaFold DB: B3QEW5

Antitoxin

• AlphaFold DB: B3QEW6