Detailed information of TA system TA229611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 31464..31989
• Replicon: plasmid pKP102-2
• Accession: NZ_CP089886
• Organism: Klebsiella pneumoniae strain KP102

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: LR951_RS27730
• Protein ID: WP_013023785.1
• Coordinates: 31684..31989 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LR951_RS27725
• Protein ID: WP_001568025.1
• Coordinates: 31464..31682 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LR951_RS27700 (LR951_27640)	26620..27399	+	780	WP_013023780.1	hypothetical protein	-
LR951_RS27705 (LR951_27645)	27675..29198	+	1524	WP_017899887.1	hypothetical protein	-
LR951_RS27710 (LR951_27650)	29232..30359	+	1128	WP_013023782.1	DUF4238 domain-containing protein	-
LR951_RS27715 (LR951_27655)	30356..30646	+	291	WP_013023783.1	hypothetical protein	-
LR951_RS27720 (LR951_27660)	30636..30833	-	198	WP_017899886.1	hypothetical protein	-
LR951_RS27725 (LR951_27665)	31464..31682	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LR951_RS27730 (LR951_27670)	31684..31989	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
LR951_RS27735 (LR951_27675)	32158..32553	+	396	WP_017899885.1	hypothetical protein	-
LR951_RS27740 (LR951_27680)	32580..32894	+	315	WP_053389906.1	hypothetical protein	-
LR951_RS27745 (LR951_27685)	32905..33921	+	1017	WP_017899884.1	hypothetical protein	-
LR951_RS27750 (LR951_27690)	34119..34913	+	795	WP_004197635.1	site-specific integrase	-
LR951_RS27755 (LR951_27695)	35361..35663	-	303	WP_004197636.1	hypothetical protein	-
LR951_RS27760 (LR951_27700)	35660..36286	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrB91 / sul1 / mph(A) / qnrS1 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16	-	1..131492	131492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T229611 WP_013023785.1 NZ_CP089886:31684-31989 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T229611 NZ_CP124498:c3590581-3590279 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT229611 WP_001568025.1 NZ_CP089886:31464-31682 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT229611 NZ_CP124498:c3590243-3589902 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8