Detailed information of TA system TA229610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 228992..229728
• Replicon: plasmid pKP102-1
• Accession: NZ_CP089885
• Organism: Klebsiella pneumoniae strain KP102

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LR951_RS27405
• Protein ID: WP_003026803.1
• Coordinates: 229246..229728 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LR951_RS27400
• Protein ID: WP_003026799.1
• Coordinates: 228992..229258 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LR951_RS27355 (LR951_27295)	225054..225416	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
LR951_RS27360 (LR951_27300)	225466..225816	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
LR951_RS27365 (LR951_27305)	226174..226443	+	270	WP_004152102.1	hypothetical protein	-
LR951_RS27370 (LR951_27310)	226431..227006	+	576	WP_004152103.1	hypothetical protein	-
LR951_RS27375 (LR951_27315)	227037..227531	+	495	WP_004152104.1	DNA-binding protein	-
LR951_RS27380 (LR951_27320)	227575..227943	+	369	WP_004152105.1	hypothetical protein	-
LR951_RS27385 (LR951_27325)	227977..228180	+	204	WP_004152106.1	HHA domain-containing protein	-
LR951_RS27390 (LR951_27330)	228229..228486	+	258	WP_004152107.1	hypothetical protein	-
LR951_RS27395 (LR951_27335)	228562..228816	+	255	WP_004152108.1	hypothetical protein	-
LR951_RS27400 (LR951_27340)	228992..229258	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LR951_RS27405 (LR951_27345)	229246..229728	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LR951_RS27410 (LR951_27350)	229936..231282	+	1347	WP_077253535.1	ISNCY family transposase	-
LR951_RS27415 (LR951_27355)	231331..231726	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
LR951_RS27420 (LR951_27360)	231874..233039	-	1166	Protein_246	IS3 family transposase	-
LR951_RS27425 (LR951_27365)	233216..234178	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
LR951_RS27430 (LR951_27370)	234165..234653	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / tet(A) / qnrB1 / dfrA14	-	1..247180	247180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T229610 WP_003026803.1 NZ_CP089885:229246-229728 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T229610 NZ_CP124498:2767497-2767604 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT229610 WP_003026799.1 NZ_CP089885:228992-229258 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT229610 NZ_CP124498:c2767440-2767385 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66