Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4670377..4671187 Replicon chromosome
Accession NZ_CP089884
Organism Klebsiella pneumoniae strain KP102

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LR951_RS22920 Protein ID WP_004178461.1
Coordinates 4670377..4670910 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LR951_RS22925 Protein ID WP_002887278.1
Coordinates 4670921..4671187 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LR951_RS22915 (LR951_22875) 4669208..4670329 + 1122 WP_002887282.1 cupin domain-containing protein -
LR951_RS22920 (LR951_22880) 4670377..4670910 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LR951_RS22925 (LR951_22885) 4670921..4671187 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LR951_RS22930 (LR951_22890) 4671290..4672723 - 1434 WP_023159196.1 Cu(+)/Ag(+) sensor histidine kinase -
LR951_RS22935 (LR951_22895) 4672713..4673396 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LR951_RS22940 (LR951_22900) 4673568..4674953 + 1386 WP_023159195.1 efflux transporter outer membrane subunit -
LR951_RS22945 (LR951_22905) 4674971..4675315 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T229606 WP_004178461.1 NZ_CP089884:c4670910-4670377 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T229606 NZ_CP124498:1927972-1928226 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT229606 WP_002887278.1 NZ_CP089884:c4671187-4670921 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT229606 NZ_CP124498:1927724-1927975 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References