Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4048466..4049085 Replicon chromosome
Accession NZ_CP089884
Organism Klebsiella pneumoniae strain KP102

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LR951_RS19895 Protein ID WP_002892050.1
Coordinates 4048867..4049085 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LR951_RS19890 Protein ID WP_002892066.1
Coordinates 4048466..4048840 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LR951_RS19880 (LR951_19845) 4043618..4044811 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LR951_RS19885 (LR951_19850) 4044834..4047980 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LR951_RS19890 (LR951_19855) 4048466..4048840 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LR951_RS19895 (LR951_19860) 4048867..4049085 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LR951_RS19900 (LR951_19865) 4049244..4049810 + 567 WP_023158101.1 maltose O-acetyltransferase -
LR951_RS19905 (LR951_19870) 4049782..4049922 - 141 WP_004147370.1 hypothetical protein -
LR951_RS19910 (LR951_19875) 4049943..4050413 + 471 WP_002892026.1 YlaC family protein -
LR951_RS19915 (LR951_19880) 4050388..4051839 - 1452 WP_023158103.1 PLP-dependent aminotransferase family protein -
LR951_RS19920 (LR951_19885) 4051940..4052638 + 699 WP_002892021.1 GNAT family protein -
LR951_RS19925 (LR951_19890) 4052635..4052775 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LR951_RS19930 (LR951_19895) 4052775..4053038 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T229605 WP_002892050.1 NZ_CP089884:4048867-4049085 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T229605 NZ_CP124498:1927724-1927975 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT229605 WP_002892066.1 NZ_CP089884:4048466-4048840 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT229605 NZ_CP124498:1927972-1928226 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References