Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 174874..175784 Replicon plasmid pKP059
Accession NZ_CP089883
Organism Klebsiella pneumoniae strain KP059

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag LVQ69_RS26610 Protein ID WP_004144067.1
Coordinates 174874..175344 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag LVQ69_RS26615 Protein ID WP_004181895.1
Coordinates 175341..175784 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ69_RS26590 (LVQ69_26555) 170618..171598 - 981 WP_085013577.1 IS5-like element IS5 family transposase -
LVQ69_RS26595 (LVQ69_26560) 171917..173020 - 1104 WP_235353125.1 Hok/Gef family protein -
LVQ69_RS26600 (LVQ69_26565) 173623..173781 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
LVQ69_RS26605 (LVQ69_26570) 174504..174764 + 261 WP_004026352.1 hypothetical protein -
LVQ69_RS26610 (LVQ69_26575) 174874..175344 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
LVQ69_RS26615 (LVQ69_26580) 175341..175784 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
LVQ69_RS26620 (LVQ69_26585) 175885..177156 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
LVQ69_RS26625 (LVQ69_26590) 177156..177578 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
LVQ69_RS26630 (LVQ69_26595) 178886..179917 - 1032 WP_029505552.1 hypothetical protein -
LVQ69_RS26635 (LVQ69_26600) 179922..180629 - 708 WP_004181864.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..262094 262094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T229592 WP_004144067.1 NZ_CP089883:c175344-174874 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T229592 NZ_CP124495:c4114892-4114605 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT229592 WP_004181895.1 NZ_CP089883:c175784-175341 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT229592 NZ_CP124495:c4114618-4114316 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References